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内源性结缔组织生长因子介导转化生长因子β_1对肾小管上皮细胞的转分化效应 被引量:4

Effects of endogenous connective tissue growth factor on renal tubular epithelial- myofibroblast transdifferentiation regulated by transforming growth faltor β_1 in vitro
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摘要 目的:探讨内源性结缔组织生长因子(CTGF)在人肾小管上皮细胞(HK2)转分化过程中的作用。方法:将HK2细胞分为4组:对照组;转化生长因子β1(TGF-β1)5ng/ml刺激组;TGF-β15ng/ml刺激+CTGF反义ODN3mmol/L干预组;TGF-β15ng/ml刺激+CTGF正义ODN3mmol/L对照组。用倒置显微镜观察各组细胞的形态学变化,用RT-PCR检测各组细胞中CTGF、上皮细胞钙粘蛋白(E-cadherin)、α-平滑肌肌动蛋白(α-SMA)、纤连蛋白(FN)mRNA表达变化。结果:TGF-β1刺激使HK2细胞形态由椭圆形转为梭形;下调E-cadherinmRNA表达,上调CTGF、α-SMA和FNmRNA表达,且CTGFmRNA表达变化在时间上早于E-cadherin、α-SMA和FNmRNA表达变化;CTGF反义ODN可对抗TGF-β1引起的细胞形态学改变和E-cadherin、CTGF、α-SMA、FNmRNA表达变化效应。结论:内源性CTGF介导了TGF-β1对HK2细胞的转分化效应。 Objective: To investigate the significance of endogenous connective tissue growth factor (CTGF) in epithel ial- myofibroblast transdifferentiation of human renal tubular epithelial cell line (HK2) in vitro. Methods:Cultured HK2 were divided into four groups: ( 1 )control; (2)treated with TGF-β1 (5 ng/ml) ; (3)treated with TGF-β1 (5 ng/ml) plus CTGF antisense oligonucleotide (3 mmol/L); (4)treated with TGF-β1 (5 ng/ml) plus CTGF sense oligonucleotide (3 mmol/L). The cell morphological changes were traced with inverted microscope, and the expression of CTGF.E-cadherin.α-smooth muscle actin (α-SMA) and fibronectin(FN) mRNAs were detected by RT-PCR. Results: In HK2 cells, TGF-β1 stimulated the oval-to-fusiform transdifferentiation in morphology, downregulated E-cadherin mRNA expression, upregulated CTGF, α-SMA and FN mRNAs expression. The presence of the change of CTGF mRNA expression preceded those of E-cadherin.α-SMA and FN mRNAs; CTGF antisense oligonucleotide inhibited these effects by TGF-β1. Conclusion:Endogenous CTGF mediates the regulation of transforming growth factor β1 in human renal tubular epithelial-myofibroblast transdifferentiation in vitro.
出处 《南京医科大学学报(自然科学版)》 CAS CSCD 北大核心 2006年第2期77-81,85,共6页 Journal of Nanjing Medical University(Natural Sciences)
基金 国家自然科学基金资助项目(30100081)
关键词 结缔组织生长因子 转化生长因子Β 肾小管上皮细胞 转分化 connective tissue growth factor transforming growth factor β renal tubular epithelial cell transdifferentiation
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  • 1姚刚,许宏岳,方奕,武晓春.1,25(OH)_2D_3对大鼠单侧输尿管梗阻肾组织核因子κB表达的影响及意义[J].南京医科大学学报(自然科学版),2005,25(10):703-705. 被引量:4
  • 2Becker GJ,Hewitson TD.The role of tubulointerstitial injury in chronic renal failure[J].Curr Opin Nephrol Hypertens,2000,9(2):133-138.
  • 3Zhang C,Meng X,Zhu Z,et al.Role of connective tissue growth factor in renal tubular epithelial-myofibroblast transdifferentiation and extracellular matrix accumulation in vitro[J].Life Sci,2004,75(3):367-379.
  • 4陆超,陈吉庆,吴升华,池霞,潘晓勤,费莉,郭梅,黄松明,郭锡熔,陈荣华.缺氧缺血对新生大鼠海马Par-4基因表达的影响及其与caspase-3活性的相关性研究[J].中华儿科杂志,2004,42(7):534-534. 被引量:2
  • 5Blom IE,Goldschmeding R,Leask A.Gene regulation of connective tissue growth factor:new targets for antifibrotic therapy[J] ? Matrix Biol,2002,21 (6):473 -482.
  • 6Abreu JG,Ketpura NI,Reversade B,et al.Connective tissue growth factor (CTGF) modulates cell signalling by BMP and TGF-beta[J].Nat Cell Biol,2002,4:599-604..
  • 7Grotendorst GR,Duncan MR.Individual domains of connective tissue growth factor regulate fibroblast proliferation and myofibroblast differentiation[J].FASEB J,2005,19(7):729-738.
  • 8Nadia AW,Roger MM.Connective tissue growth factor and renal diseases:some answers,more questions[J].Curr Opin Nephrol Hypertens,2004,13:53-58.
  • 9Okada H,Kikuta T,Kobayashi T,et al.Connective tissue growth factor expressed in tubular epithelium plays a pivotal role in renal fibrogenesis[J].J Am Soc Nephrol,2005,16(1):133-143.
  • 10Kalluri R,Neilson EG.Epithelial-mesenchymal transition and its implications for fibrosis[J].J Clin Invest,2003,112(12):1776-1784.

二级参考文献16

  • 1Gozal E, Gozal D, Pierce WM, et, al.Proteomic analysis of CA1 and CA3 regions of rat hippocampus and differential susceptibility to intermittent hypoxia. J Neurochem,2002,83:331-345.
  • 2Barradas M, Monjas A, Diaz-Meco MT, et al.The downregulation of the proapoptotic protein par-4 is critical for Ras induced survival and tumor progression. EMBO J,1999,18: 6362-6369.
  • 3Boehrer S, Chow KU, Beske F, et, al.In lymphatic cells par-4 sensitizes to apoptosis by down-regulating bcl-2 and promoting disruption of mitochondrial memberane potential and caspase activation. Cancer Res, 2002, 62:1768-1775.
  • 4Panichi V, Migliori M, T accola D, et al. Effects of calcitriol on the immune system: new possibilities in the treatment of glomerulonephritis [J]. Clin Exp Pharmacol Physio, 2003,30(11): 807-811.
  • 5Tanaci N, Karakose H, Guvener N, et al. Influence of 1,25-dihydroxyvitamin D3 as an immunomodulator in renal transplant recipients: a retrospective cohort study [J]. Transplant Proc, 2003 ,35(8):2885-2887.
  • 6Weinreich T, Merke J, Schonermark M, et al. Actions of 1,25-dihydroxycitamin D3 on human mesangial cells [J]. Am J Kidney Dis , 1991,18(3):359-366.
  • 7Weih M, Orth S, Weinreich T,et al. Inhibition of growth by calcitriol in a proximal tubular cell line [J]. Nephrol Dial Transplant, 1994,9(9): 1390-1395.
  • 8Panichi V, Migliori M, Taccola D, et al. Effects of 1,25in experimental mesangial proliferative nephritis in rats [J]. Kidney Int, 2001,60(1): 87-95.
  • 9Redaelli CA, Wagner M, Gunter-Duwe D,et al. lα,25-Dihydroxyvitamin D3 shows strong and additive immunomodulatory effects with cyclosporine A in rat renal allotransplants [J]. Kidney Int, 2002,61(1):288-296.
  • 10Schwarz U, Amann K, Orth SR ,et al. Effect of 1,25(OH)2 vitamin D3 on glomerulosclerosis in subtotally nephrectomized rats [J]. Kidney Int, 1998,53(6): 1696-1705.

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