摘要
目的:寻找诊断华支睾吸虫病的特异性抗原。方法:应用SDS-PAGE和Western blot对华支睾吸虫成虫可溶性抗原中的蛋白组分进行分析,试验血清包括旋毛虫感染的大鼠和小鼠血清,正常大鼠和小鼠血清,旋毛虫病、华支睾吸虫病、并殖吸虫病、日本血吸虫病及囊虫病患者血清,斯氏狸殖吸虫感染的大鼠血清和正常人血清。结果:华支睾吸虫成虫可溶性抗原蛋白经SDS-PAGE后显示17条蛋白带,其中相对分子质量为65000、54000、31000、25000、13000的为主带。Western blot结果显示,相对分子质量为108000、94000、71000、65000、56000、53000、43000的蛋白带可被华支睾吸虫病患者血清识别,108000、71000、65000、63000、53000的蛋白带可被并殖吸虫病患者血清识别,108000、94000、71000可被日本血吸虫病患者血清、旋毛虫感染的大鼠血清及旋毛虫病患者血清识别;56000的蛋白组分只能被华支睾吸虫病患者血清识别,而不与其他试验血清发生交叉反应。结论:华支睾吸虫成虫可溶性抗原中相对分子质量为56000的蛋白组分为华支睾吸虫成虫的特异性抗原。
Aim： To find out the specific antigens for immunodiagnosis of clonorchiasis. Methods： The soluble antigens of Clonorchis sinensis（C.sinensis）adult worms wrer analyzed by SDS-PACE and Western blot. Sera for this study included sera from patients with clonorchiasis,paragonimiasis,schistosomiasis,trichinellosis,sera from rats and mice infected with T.spicalis,and sera from normal rats, mice and persons. Results：SDS-PAGE revealed that the soluble antigens of C.sinensis adult worms had 17 protein bands.Westerm blot results showed that the protein bands with MW 108 000, 71000,65000,63000,53000 were crose-reacted with sera from patients with paragonimiasis. All of the protein bands with MW 108000,94000,71000 were cross-reacted with sera from patients with schistosomiasis, rats infected ith T.spiralis and patients with trichinellosis. The pratein component with MW 56000 was only reacted with sera from patients with clonorchiasis.Conclusion：The protein component with MW 56000 is the specific antigen for C.sinensis adult worm.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2006年第1期80-82,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关计划基金资助项目991170411
关键词
华支睾吸虫
抗原
成虫
Clonorchis sinensis
antigen
adult worm
