摘要
目的检测构建的PcDNA3.1/sTNFRⅠ真核表达载体在体外转染中国仓鼠卵巢(CHO)细胞后能否有效表达目的产物。方法ELISA法检测PcDNA3.1/sTNFRⅠ转染的CHO细胞培养上清液中sTNFRⅠ的表达。结果重组质粒转染的CHO细胞上清液中表达的sTNFRⅠ量超过1 000 pg/mL,显著高于对照组(109.6±5.32)pg/mL。结论PcDNA3.1/sTNFRⅠ重组质粒在体外导入哺乳动物细胞后能够表达相应的目的产物。
Objective To detect the expression of recombinant plasmid PcDNA3.1/sTNFR Ⅰ in vitro. Methods CHO ceils were transfected with recombinant plasmid PcDNA3.1/sTNFRⅠ by lipesome, sTNFR Ⅰ expressed in cell culture supematant was detected by ELISA. Results It was much higher of sTNFR Ⅰ in transfected cell culture supernatant than control groups. Conclusion These results showed that the recombinant plasmid PcDNA3.1/sTNFR Ⅰ can be expressed in mammalian cells.
出处
《广东牙病防治》
2006年第1期13-15,共3页
Journal of Dental Prevention and Treatment
基金
广东省医学科学技术研究基金资助项目(A2004119)
