摘要
目的研究选择性COX-2抑制剂对HGF诱导的肝癌细胞株增殖及MMP-2、TIMP-2 mRNA表达的影响,探讨药物与肿瘤转移之间的关系。方法肿瘤细胞培养于不含有和含有30ng/ml HGF的2%FBS DMEM培养基中,MTT法检测NS-398对肝癌细胞株增殖的影响,RT-PCR法检测NS-398对肝癌细胞株MMP-2T、IMP-2 mRNA表达的影响。结果在含有30ng/ml HGF的2%FBS DMEM培养基中肿瘤细胞出现数量增多。浓度低于60μmol/L的NS-398不影响HGF诱导的肝癌细胞株的增殖,但抑制细胞内MMP-2、TIMP-2 mRNA表达。浓度高于90μmol/L的NS-398抑制肝癌细胞株的增殖。结论选择性COX-2抑制剂能够上调肝癌细胞株内TIMP-2/MMP-2的mRNA比值,并可能具有降低肿瘤细胞运动性的潜在作用,且具有抑制肿瘤细胞增殖的作用。
Objective To study the effect of selective COX-2 inhibitor upon HGF induced proliferation of hepatoearcinoma cell line and the expression of MMP-2 and TIMP-2 mRNA, and to verify the relationship between drug interference and cancer metastasis. Methods HepG2 cells were cultured in 2% FBS enriched DMEM medium with or without 30ng/ml HGF addition. MTT test and RT-PCR were employed to demonstrate the influence of NS-398 upon cell line proliferation and MMP-2 and TIMP-2 rnRNA expression, respectively. Results 30ng/ml HGF benefits proliferation of HepG2 cells growing in 2% FBS enriched DMEM. NS-398 had no effect on HepG2 cell proliferation at concentrations below 60μmol/L, but did inhibit the expression of MMP-2 and TIMP-2 mRNA, while when its concentration was above 90mmol/L, the proliferation of HepG2 cell was obviously reduced. Conclusion Selective COX-2 inhibitor can up-regulate TIMP-2/MMP-2 mRNA ratio of HepG2 cell, and may probably have the potential to reduce the mobility of cancerous cells. Further, selective COX-2 inhibitor may have inhibitory effect upon cancerous cell proliferation.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2006年第1期30-32,共3页
Journal of Harbin Medical University
