摘要
目的研究大肠癌VEGFR3、podoplanin和CD34阳性脉管的特点及其与转移的关系。方法采用双标及单标免疫组化方法分析96例大肠癌及其相应正常大肠组织VEGFR3、podoplanin和CD34阳性脉管的特点,并分别评估其预测大肠癌转移的价值。结果大肠癌边缘区VEGFR3、podoplanin和CD34阳性脉管密度显著高于其他区域(P<0.01、0.05),也显著高于正常大肠组织(P<0.01)。VEGFR3阳性脉管与podoplanin或CD34阳性脉管有交叉。大肠癌转移与肿瘤边缘podoplanin和CD34阳性脉管密度密切相关(P<0.01、0.05)。结论VEGFR3阳性脉管既有淋巴管也包含血管;血管密度,尤其肿瘤边缘淋巴管密度是预测大肠癌转移的有效参考指标。
Objectives To study the characteristics of VEGFR-3, podoplanin or CD34 positive vessels in colorectal cancer （CRC） and their correlation with metastasis. Methods The characteristics of VEGFR-3, podoplanin or CD34 positive vessels in 96 cases with CRC and normal mucosa were evaluated by single-labeling or double-labeling immunohistochemistry with anti-human VEGFR-3, podoplanin or C1334 antibody respectively, and their relationship with metastasis of the cancer was analyzed. Results The density of VEGFR-3, podoplanin or CD34 positive vessels at the peripheral region of CRC was significantly higher than that of other regions of CRC or that in normal mucosa （P〈0. 01 or 0. 05）. Anti-human VEGFR 3 antibody was found to have cross reaction with podoplanin or CD34. Density of podoplanin or CD34 positive vessels at the peripheral region of CRC was significantly correlated with metastasis （P〈0. 01 or 0. 05）. Conclusion VEGFR 3 positive vessels consist of both lymphatic and blood vessels. Density of blood vessels, especially of lymphatic vessels, located at the peripheral region of CRC may serve as a useful marker to predict metastasis.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2006年第1期64-66,共3页
Medical Journal of Chinese People's Liberation Army
