摘要
目的建立和优化终末期肾病患者血清蛋白质组研究的双向电泳及相关技术,并与正常血清蛋白图谱比较。方法以固相pH梯度等电聚焦为第一向和垂直SDS-聚丙烯酰胺凝胶电泳为第二向,对终末期肾病患者血清蛋白质样品制备、上样量、IPG胶条、等电聚焦等进行条件优化。质谱鉴定有差异表达的其中4个蛋白点。结果成功获得终末期肾病患者血清蛋白质组电泳图谱,与正常者相比存在表达有明显差异的蛋白点。结论成功建立了终末期肾病患者血清蛋白质组研究的双向电泳技术平台。
Objective To establish and optimize the two-dimensional gel electrophoresis technical platform for the blood serum proteome research in patients with end stage renal disease （ESRD）. Methods Immohiline pH gradients isoelectric focusing was used as the first dimensional gel electrophoresis and the vertical SDS-PAGE was used as the second dimensional gel electrophoresis （2-DE）. The 4 differentially expressed protein spots were identified by mass spectrometry. Results Satisfactory 2-DE maps of ESRD patients serum protein were obtained and there were some differentially protein spots between the 2-DE maps of ESRD patients and normal controls. Conclusions The 2-DE technology for the serum proteome of ESRD patients is set up.
出处
《中国医师杂志》
CAS
2006年第1期37-39,共3页
Journal of Chinese Physician
关键词
肾功能衰竭
蛋白质组
电泳
凝胶
双向
Kidney failure
Proteome
Electrophoresis, gel, tow dimensional
