摘要
目的建立一种新的小鼠气道上皮杯状细胞增生和粘液高分泌模型。方法用卵白蛋白加氢氧化铝凝胶皮下注射致敏小鼠，d10腹腔注射重复致敏1次，然后从第1次致敏后d15～21每天用卵白蛋白雾化吸入攻击1次。检测AB-PAS染色的支气管上皮杯状细胞数目，HE染色的肺组织嗜酸性粒细胞浸润和支气管灌洗液中的炎症细胞数目，并用已知有效药物和未知受试药物验证此模型。结果卵白蛋白致敏和攻击的小鼠呈现明显的支气管上皮杯状细胞增生，管腔内黏液增多，肺组织嗜酸性粒细胞浸润和支气管灌洗液中的炎症细胞数目增加，这些病理改变可被地塞米松和小鼠IL-12质粒抑制。结论这一模型有助于筛选抗黏膜高分泌的新药和研究气道黏膜高分泌的病理机制。
Aim Establish a novel mouse model of airway goblet cell hyperplasia and mucus hyperseeretion. Method BALB/c mice were sensitized subcutaneously with ovalbumin （ OVA ） allergens in aluminium hydroxide at d 0, re-sensitized once intraperitoneally d 10 after first sensitization, and challenged with OVA aerosolly dally from d 15 to d 21 after first sensitization. Inflammatory cell number in BALF, eosinophil infiltration in the lung tissue with HE stain and goblet cells in the bronchial mucous membrane with PAS stain were examined. Dexamethasone was employed to validate the model. Results Mice sensitized and challenged with OVA showed a significantly hyperplasia of goblet cells in the bronchial mucous membrane, increased mucus in the alveolar cavity, eosinophil infiltration in the lung tissue and increased number of inflammatory cells in BALF. Those pathological changes were inhibited by dexamethasone and mIL-2 plasmid. Conclusion This model helps to screen the new diugs for mucus hypersecretion and research on the pathological mechanism of airway mucus hypersecretion.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2006年第2期251-253,共3页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30371314)
浙江省科学技术厅项目(No2003c33004)
关键词
小鼠
杯状细胞
粘液高分泌
气道
mouse
goblet cell
mucus hypersecretion
airway
