摘要
目的研究硒螺旋藻(Se-SP)对大鼠肝叶切除术后肝细胞再生和抗氧化能力的影响,以印证其生物利用价值。方法用W istar大鼠复制6 7%肝叶切除动物模型,设Se-SP高剂量(H)和低剂量(L)治疗组,术前以Se-SP灌胃7 d;另设生理盐水(C)及假手术对照组(S)。于手术前及其后2 4 h取鼠肝细胞,检测硒(Se)、丙二醛(MDA)含量及谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)活性;利用免疫组化方法测定肝细胞增生核抗原(PCNA)表达指数,慧星实验检测过氧化氢诱导的肝细胞DNA损伤。结果与C,S组相比,H组、L组肝细胞中MDA水平明显降低(P<0.0 5),而Se,GPx和SOD明显升高(P<0.0 5);肝细胞PCNA指数明显升高(P<0.0 5),过氧化氢诱导的DNA损伤程度较轻。结论Se-SP对肝切除鼠肝细胞具有一定的抗氧化和促再生作用。
Objective To explore the effects of selenium enriched Spirulina platensis （Se-SP） on antioxidation and regeneration of rat hepatoeytes after partial hepatectomy. Methods Sixty-seven percent hepatectomy was performed in rats after high dose （ H ） and low dose （ L ） Se- SP was given via gastric tube for 7 days. Then selenium （Se）, glutathione peroxidase （GPx）, superoxide dismutase （SOD） and malondiaoldehyde （MDA） in hepatocytes were determined. The results were compared with normal saline giving via gastric tube before hepatectomy （C） and sham operation （S） groups. Hepatocytes were obtained before operation and 24h after operation, and the expression index of proliferating cell nuclear antigen （ PCNA ） in hepatocytes was detected by immunohistochemistry, and the DNA damage of hepatocytes induced by peroxide was analyzed by comet assay. Results After partial hepatectomy, Se content, GPx and SOD activity, and PCNA expression index were obviously higher （ P 〈 0. 05 ） , whereas content of MDA was significantly lower （ P 〈 0.05 ） in hepatocytes of rats with high dose and low dose Se-SP（ 150mg/100/ d） than those in control. The DNA oxidation damage in hepatocytes induced by peroxide （100 nM for 30 min ） was decreased also in rats with high dose and low dose of Se-SP treatment. Conclusions Se-SP has definite antioxidation and regeneration enhancing effects on rat hepatocytes after hepatectomy.
出处
《中国普通外科杂志》
CAS
CSCD
2006年第1期19-22,共4页
China Journal of General Surgery
基金
广东省自然科学基金重点资助项目(05103295)
关键词
肝切除术
肝再生
硒螺旋藻
Hepatectomy
Liver Regeneration
Se- SP
