摘要
目的探讨银杏叶提取物(EGb)预处理对大鼠移植肝的保护作用。方法采用Kamada′s袖套法建立大鼠原位肝移植模型。将大鼠随机分为银杏叶提取物预处理(EGb)组、生理盐水对照(NS)组和假手术组(SO)。分别于供肝再灌注后2,6,2 4 h处死动物,检测血清ALT和AST;肝组织组织学检查,TUNEL法检测细胞凋亡;RT-PCR检测肝组织TNF-αmRNA及Bcl-2mRNA的表达。结果供肝再灌注2,6,2 4 h,EGb组血清ALT水平及细胞凋亡指数均明显低于生NS组(P<0.0 1)。血清AST水平在供肝再灌注2,6 h时明显低于NS组(P<0.0 1)。供肝再灌注后2,6 h时EGb组TNF-αmRNA的表达明显低NS组(P<0.0 5)。供肝再灌注后2,6,2 4 hEGb组Bcl-2mRNA的表达明显高于NS组(P<0.0 1)。结论经EGb预处理供可减轻大鼠肝移植供肝的缺血/再灌注损伤和细胞凋亡,影响TNF-αmRNA,Bcl-2mRNA的表达,对供肝有保护作用。
Objective To investigate the protective effects of Ginkgo Biloba leaves （ EGb ） preconditioning on liver graft in rat liver transplantation. Methods Male Sprague-Dawley rats were used as donors and recipients of orthotopie liver transplantation （ OLT ） . The rats were randomly divided into EGb group, normal saline（NS） control group and sham operation（SO） group. The animals were killed at 2h, 6h, 24h after graft reperfusion. Plasma samples were collected for ALT and AST test. Liver tissues were collected to detect the expression of TNF-α mRNA and Bcl-2mRNA by RT-PCR. Also, liver tissues were used to detect rat liver histological change and apoptosis by TUNEL. Results The serum levels of ALT in EGb group were significantly lower than the NS group（ P 〈 0.01 ） at 2h, 6h and 24h after reperfusion. The serum levels of AST in EGb group were significantly lower than the NS group （ P 〈 0. 01 ） at 2h and 6h after reperfusion. The apoptosis index （ AI ） of liver tissue in EGb group was significantly lower than the NS group （ P 〈 0.01 ） at 2h, 6h and 24h after reperfusion. The expression of TNF-α mRNA was significantly lower in EGb group than the NS group（ P 〈 0.05 ） at 2h and 6h after reperfusion. The expression of Bcl-2 mRNA was significantly higher in EGb group than the NS group （ P 〈 0.01 ） at 2h, 6 h and 24h after reperfusion. Conclusions Preconditioning with extracta of Ginkgo Biloba leaves can attenuate liver graft ischemia/ reperfusion injury and apoptosis of rat hepatocytes by affecting the expression of TNF-α mRNA, and Bcl-2 mRNA and exert a protective effect on the liver graft.
出处
《中国普通外科杂志》
CAS
CSCD
2006年第1期26-31,共6页
China Journal of General Surgery
关键词
肝移植
再灌注损伤/预防和控制
银杏叶提取物
Liver Transplantation
Reperfusion Injury/prey
Extracta of Ginkgo Biloba Leaves
