补阳还五汤提取液对Schwann细胞氧化损伤的保护作用被引量：27

THE PROTECTIVE EFFECTS OF BUYANGHUANWU DECOCTION ON THE OXIDATIVE INJURIED SCHWANN CELL

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摘要本实验建立了Schwann细胞的体外氧化损伤模型,从对Schwann细胞的抗氧化损伤作用探讨补阳还五汤的疗效机制。用原代培养的Schwann细胞建立氧化损伤模型,将培养细胞分为氧化损伤组,补阳还五汤处理组及正常组:(1)应用MTT方法检测细胞的活性;(2)应用生化技术检测细胞内超氧化物歧化酶(SOD)的含量;(3)采用荧光探针Fluo-3-AM标记细胞,用激光共聚焦显微镜观察H_2O_2对Schwann细胞内游离钙([Ca2+])抗氧化损伤的影响。结果显示:与对照组相比,H2O2处理组细胞活性降低(P<0.01),SOD含量明显减少(P<0.01),而补阳还五汤预处理后细胞内[Ca2+]i升高明显被减弱(P<0.01),细胞存活率明显升高(P<0.01)。以上结果表明补阳还五汤能通过抗过氧化损伤保护Schwann细胞。 To investigate the protective effects of Buyanghuanwu Decoction on the oxidative injuried Schwann cell;after making the oxidative injuried model of the cultured Schwann cell ：（ 1 ） MTT was used to observe cell activity of the Schwann cell treated with hydrogen peroxide; （2） Detecting the intracellular superoxide dismutase （SOD） ; （3） The fluorescent probe Fluo-3-AM was used to detect the intracellular Ca^2＋ levels by using Lasers Scanning Confocal Microscopy （LSCM）. The results showed that, compared with the control group, the oxidative injured Schwann cells had decreased cell activity and elevated intracellular Ca^2＋ level. However, these changes were significantly inversed by Buyanghuanwu Decoction preconditioned group. These results suggest that Buyanghuanwu Decoction has good antioxidated effect to protect the Schwann cells.

作者邢三丽李振华孙晋浩刘岳鹏暴丽华

机构地区山东大学医学院解剖学教研室

出处《神经解剖学杂志》 CAS CSCD 北大核心 2006年第1期95-98,共4页 Chinese Journal of Neuroanatomy

基金山东省自然科学基金(Y2000C14) 山东省优秀中青年科学家科研奖励基金(2004BS02012)资助项目

关键词 SCHWANN细胞氧化损伤抗氧化作用补阳还五汤 Schwann cell, oxidated damage, antioxidated effect, Buyanghuanwu Decoction

分类号 R285 [医药卫生—中药学]

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