摘要
以渤海和黄海分离出400多株在低温条件下生长良好的菌株为出发菌株,利用常规筛选方法选出2株低温蛋白酶产生菌(Pseudom onas alcaligenes)。经UV、DES、NTG、EMS、L iC l单独及复合诱变,选育出一株(Pa040523)蛋白酶高产突变株。通过单因素实验,确定了Pa040523菌株蛋白酶发酵培养基为:玉米淀粉糖1.8%,尿素0.6%,磷酸氢二钾0.6%,磷酸二氢钾0.3%。该突变株低温蛋白酶产量为940.8 U/mg。
Two Marine low temperature acid protease producing strains were obtained from more than 400 Pseudamoral, which isolated from marine. A marine low temperature acid protease producing mutant （Pa040523） was bred through multiple mutagenesis （ UV, DES and NTG etc） . Through orthogonal experiment, the optimal condition was given for producing marine low temperature, acid protease of Pa040523. The suitable medium was that corn syrup, 1.8 % ; urea, 0.6% ; K2HPO4, 0.6% ; KH2PO4, 0. 3 %, The production of marine low temperature acid protcase was 940. 8 U/mg.
出处
《微生物学通报》
CAS
CSCD
北大核心
2006年第1期114-117,共4页
Microbiology China
