摘要
通过选择性富集培养，从沈抚灌区石油污染土壤中分离到1株菲降解细菌L2，该菌株能以菲为唯一碳源和能源生长。通过对L2菌株培养条件优化，确定其最佳培养条件为pH值7．0，7．2。温度28～30℃，150ml容积三角瓶装液量50ml。并测定了12菌株对不同浓度菲的降解率，结果表明，在培养7d后L2菌株对培养基中浓度为50、100、150mg/ml的菲的降解率分别为100％、96．4％和97．4％。
A phenanthrene-degrading strain L2 was isolated from oil-contamited soil in Shenfu irrigation area by selective enrichment culture. The strain was able to grow on phenanthrene as a sole carbon and energy source for growth, it was suggested that the optimization of culture condition was： pH 7.0 - 7.2,28 - 30℃, 50 ml inventory/150 ml flask. Meanwhile, the degradation rate of phenanthrene under different phcnanthrene concentrations was determined. It was shown that after 7 days of cultivation, the degradation rate under phenanthrene concentrations of 50 mg/ml, 100 mg/ml, 150 mg/ml were 100% .96.4% and 97.4% respectively.
出处
《安徽农业科学》
CAS
北大核心
2006年第3期407-408,共2页
Journal of Anhui Agricultural Sciences
基金
国家863项目(2004AA649060)
中国科学院陆地生态过程重点实验室开放课题
关键词
菲
降解率
培养条件
Phenanthrene
Culture condition
Biodegradation
