摘要
目的 研究A型肉毒毒素肌注后神经芽牛的病理和电生理过程。方法 SD大鼠40只，随机分为肉毒毒素组和生理盐水组。在SD大鼠右侧腓肠肌肌注5U的A型肉毒毒素或同容积的生理盐水，于肌注后1，4，8，12周测定单纤维肌电图的运动电位平均连续波间差（MCD）和肌纤维密度，观察病理神经纤维数目。结果 运动单位MCD结果为1～4周显著延长，8周开始好转，12周恢复正常；提示肌注肉毒毒素后出现神经肌肉接头传导阻滞，并于12周恢复功能。肌纤维密度和神经纤维数目计数均增加，结果 提示肌注肉毒毒素后出现神经芽生。结论 大鼠肌注肉毒毒索后出现了神经芽生，神经肌肉接头功能在12周内恢复。
Objective To investigate the pathological and electro-physiological process of terminal sprouting after muscle injection of Botulinum toxin type A （BTXA） in rats. Methods Sprague-Dawley rats were randomly divided into a BTXA group and a control group. Five unit of BTXA was injected into the right gastrocnemius of the rats in BTXA group, instead of 0.9% saline in the control group. The morphologic axon analysis and SFEMG （mean consecutive difference and fiber density） were measured at 1,4,8,12 week after injection. Results The mean consecutive difference was prolonged in 1-4 week, and got better at 8 week, recovered at 12 week. It was revealed of the recovery of neuromuscular junction at 12 week after BTXA injection. The results of fiber density and morphologic axon analysis demonstrated terminal sprouting after injection of BTXA. Conclusion Motor nerve terminal sprouting could appear after BTXA injection and the function of neuromuscular junetion could become recovered at 12 week.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2006年第2期98-101,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
A型肉毒毒素
神经肌肉接头
神经芽生
单纤维肌电图
Botulinum toxin type A
Neuromuscular junction
Terminal sprouting
Single fiber electromyography
