摘要
目的研究纳米As_2O_3磁性脂质体联合磁感应加热治疗异种移植性宫颈癌的作用及机制。方法建立裸鼠人宫颈癌移植瘤模型,将纳米As_2O_3脂质体、纳米磁性脂质体和纳米As_2O_3磁性脂质体等分别局部注射到裸鼠人宫颈癌移植瘤中,交变磁场(AMF)作用30d后检测抑瘤率、裸鼠移植瘤凋亡、增殖指数、Bcl-2和Bax蛋白表达及其对肝、肾功能的影响等。结果在AMF作用下,各组移植瘤的质量抑制率分别为57.06%(P<0.05)、49.44%(P<0.05)和80.84%(P<0.01)。凋亡相关指标(AI、PI、Bcl-2、Bax)与对照组相比差异均有统计学意义(P<0.05);各组血清AST、ALT、BUN及Cr的均值之间相比,差异无统计学意义(P>0.05)。实验组肿瘤组织有明显坏死,而瘤旁和内脏组织均未见明显损伤。结论纳米As_2O_3磁性脂质体是一种较理想的靶向治疗肿瘤的复合载体,可以同时发挥药物和磁感应加热的联合定向治疗作用,且对肝、肾功能没有明显的毒性,极可能成为一种宫颈癌治疗新途径。
Objective To explore the inhibition effects and mechanics of nano-magnetoliposomes containing As2O3 （NMLA） combined with magnetic fluid hyperthermia on xenograft cervical carcinoma in nude mice. Methods After establishment of xenograft cervical carcinoma models in nude mice, nano-liposomes containing As2O （NLA group）, nano-magnetolipnsomes （NML group） and NMLA （NMLA group） were injected into the xnografl cervical carcinoma, respectively, and all nude mice were exposed to AMF for three times. The inhibitive ratios （IR） of the tumors, apoptosis index （AI） , proliferation index （PI） anti the expression of Bcl-2 and Bax proteins were measured, as well as the function of liver and kidney. Results lRs in NLA, NML and NMLA groups were 57.06% （P 〈 0.05）, 49.44% （P〈0.05） and 80.84% （P〈0.001）, respectively. Compared with those in the control group, AI and Bax protein expression in experimental groups were higher （ P 〈 0.05 ） , while PI and Bcl-2 protein expression were lower （P 〈0.05）. There was no distinct difference in the values of ALT,AST,Bun and Cr （P 〉 0.05）. The obvious necrosis was observed in the tumor tissues in NLA, NML and NMLA groups, but not in the peripheral tissues of the tumors and the visceras. Conclusion NMLA, without toxicity of liver and kidney, might be a desirable composite carrier of targeted treatment with chemotherapy and thermotherapy simultaneously.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2006年第2期102-104,共3页
Chinese Journal of Physical Medicine and Rehabilitation
基金
国家自然科学基金资助项目(No.30371830)
江苏省自然科学基金资助项目(No.BK2001003)
江苏省中医药
中西医结合重点资助项目(No.H027)
关键词
异种移植性宫颈癌
磁感应加热
纳米As203磁性脂质体
Xenograft cervical cancer
Radiofrequeney-induced hyperthermia
Nano-magnetoliposomes with As2O3
