摘要
目的:调查D16S539、D7S820及D13S317三个STR基因座在张家口地区汉族人群的等位基因分布,获得相关群体遗传学参数。方法:用SilverⅢMultipiex试剂盒复合扩增,聚丙烯凝胶电泳分型,银染显带。结果:108例汉族群体在3个基因座上均各检出7个等位基因,多态性分别为0.7314、0.7371、0.7635;个人识别率依次为0.8912、0.8948、0.9223,累计0.9986。结论:3个STR基因座在本地有较高的遗传多态性,在法医学及人类遗传学方面有较好的应用价值。
Objective： To investigate the distribution of three STR olci： D16SS39,D7S820 and D13S317 in Zhangjiakou han population ,in order to get correlative genetics parameter in the group. Methodst Silver HI Multipiex Box was used to multiply polymerase chain which would reacted with these three STR loci and the types were separated with PAGE, then strips were displayed with silver coloration. Results, 7 equipotertial genes were checked in the STR loci of 108 cases. The polymorphisms were 0. 7314,0. 7371,0. 7635 respectively. The rates for individual identification were 0. 8912,0. 8948,0. 9223 and the accumulative rate was 0. 9986. Conclusion, Three STR loci had high genetic polymorphism in Zhangjiakou. It is valuable in the application of medical jurisprudence and human genetics.
出处
《河北北方学院学报（医学版）》
2005年第6期36-37,共2页
Journal of Hebei North University:Medical Edition
关键词
法医物证学
STR基因座
遗传多态性
复合扩增
Forensic biological evidence
STR locus
Genetic polymorphism
Multiplex polymerase chain reaction
