摘要
目的建立内毒素致幼鼠脑室周围白质软化(periventricular leukomalacia,PVL)的动物模型,探讨宫内感染与脑白质损害及其后遗大脑性瘫痪的内在免疫学机制。方法 26只健康 Wistar孕鼠,分为实验组和对照组。于妊娠第15天按0．3 mg／kg剂量将脂多糖(lipopdysccharide, LPS)对实验组孕鼠腹腔内注射,对照组孕鼠在同一天使用等量蒸馏水腹腔内注射,妊娠第21天,孕鼠均分娩,取实验组幼鼠40只、对照组幼鼠35只,采用放射免疫法测定两组幼鼠脑组织IL-2含量及 TNF-α含量。另将实验组幼鼠48只,对照组幼鼠35只的脑组织进行HE染色;最后将两组剩余幼鼠 (实验组40只,对照组35只)进行脑组织含水量测定。结果实验组脑组织IL-2含量[(3．56± 0．45)ng／g]及TNF-α含量[(8．23±0．47)ng／g]明显高于对照组[(0．78±0．2)ng／g,(5．23±0.32) ng／g](P<0．01);实验组脑组织含水量[左:大脑半球(89．86±1．09)％,右:大脑半球(88．94± 1.04)％]较对照组脑组织含水量[左:(88．14±1．13)％,右:(87．98±1．06)％]明显增高(P<0．01); 实验组幼鼠脑组织切片均见脑室周围白质水肿软化、带状或灶状凝固性坏死及囊腔形成,对照组幼鼠脑组织切片未见白质水肿及软化灶。结论 LPS可导致幼鼠脑室周围白质软化,且脑组织中IL-2 和TNF-α含量均明显增高;脑组织含水量也增高;深入研究细胞因子在PVL发生中的作用机制,有望为PVL及其后遗大脑性瘫痪的预防和治疗提供新的途径。
Objective To establish the animal model of periventricular leukomalacia （PVL） caused by lipopolysaccharide （LPS） and investigate the immune mechanisms in the pathogenesis of cerebral palsy due to intrauterine infection. Methods Twenty six mature pregnant Wistar rats were divided into experiment（n= 15）and control groups（n= 11）. LPS（0. 3 mg/kg） and distilled water were injeeted on 15 days of gestation to two groups respeetively intraperitoneally, One hundred and twentyeight neonatal rats of experiment group and 105 in the eontrol group were obtained till the gestation day of 21. The level of IL-2, TNF-α and histology changes in neonatal rats＇ brain were observed by HE staining and RIA, water content in brain tissues were compared. Results IL-2[（3.56±0. 450） ng/g] and TNF-α concentrations [（8. 23±0. 47）ng/g] in neonatal rats＇ brain of the experiment group were significantly higher than those in the control group [ （0.78 ± 0. 2）ng/g and （5.23 ± 0.32）ng/g, P〈0.01）]. Water content in neonatal rats＇ brain of the experiment group [left： （89.86±1. 09） and right： （88, 94±1.04） %]was higher than that in the control group [left： （88. 147±1.13）% and right： （87. 98± 1.06）%] （P〈0.01）. Periventricular leukomalaeia, band-like, spotty coagulation necrosis and cyst formulation were found in neonatal rats＇ brain in the experimental group, but not in the controls. Conclusions Lipopolysaccharide given to maternal rats eould result in periventrieular leukomalaeia, and increase the level of IL-2 ,TNF-α and water content in the brain tissues of neonatal rats. Demonstration of the role of eytokine in the pathogenesis of PVL may be helpful in the prevention and treatment of PVL and cerebral palsy.
出处
《中华围产医学杂志》
CAS
2006年第1期36-39,T0001,共5页
Chinese Journal of Perinatal Medicine
基金
黑龙江省卫生厅科研项目(2004-140)
关键词
脂多糖类
脑性瘫痪
白质软化病
脑室周围
Lipopolysaecharides
Cerebral Palsy
Periventrieular, leukomalaeia
