摘要
目的对海洋放线菌M326活性代谢产物进行初步研究。方法以抑菌活性为指标，采用不同的培养基与培养时间研究代谢产物活性与培养条件的关系，用不同温度、pH处理了解活性产物的稳定性，用有机溶剂，大孔树脂对活性物质进行提取分离。结果人工海水、蒸馏水配制的GBP培养基产物活性较强。抗菌物质在pH2～11时较稳定，强酸性条件下热稳定性好，难以用一般有机溶剂萃取，碱性条件下可被AB8大孔树脂吸附。结论代谢产物对G^-菌有较强的抑菌活性，对G^-菌、耐药菌均有不同程度的抑菌活性，且抗菌物质的极性较强。
Objective To study bioactive metabolite produced by marine actinomyces M326. Methods Based on the index of antimicrobial activity, the relation between activity of metabolite and conditions of culture in different media and time were researched , and the stability of bioactive metabolite treated with different temperatures and pH were obseved, and the bioactive ingredients were extracted and separated by organic solvent and by macroporous resin. Results The GBP medium made by artificial seawater or distilled water were suitable for production of strong bioactive metabolites. The antimicrobial substances were stable under pH 2~11 and were heat-resistant under strong acidic condition. It could not be extracted by usual organic solvent, but could be absorbed by AB8 macroporous resin under alkaline situation. Conclusion The metabolites have strong activity against gram positive bacteria and have different antimicrobial activities against gram-negative bacteria and drug-resistant strains moreover, the polarity of antimicrobial substances is strong.
出处
《中国海洋药物》
CAS
CSCD
2006年第1期44-47,共4页
Chinese Journal of Marine Drugs
关键词
海洋放线菌
活性代谢产物
抑菌活性
marine actinomyces
bioactive metabolite
antimicrobial activity
