摘要
目的通过大鼠自体原位肾移植建立肾脏缺血再灌注损伤(ischemia-reperfusion injury,IRI)模型,并观察其稳定性。方法雄性SD大鼠30只,假手术组(A组)10只,自体原位肾移植组(B组)10只,观察模型稳定性组(C组)10只。A组:不行左肾蒂血管阻断和灌注,无IRI,而单纯低温保存左肾4 h后结扎右肾蒂;B组和C组:左肾蒂血管阻断和灌注,低温保存4 h后结扎右肾蒂,开放血流。A组和B组取血和左肾检查,C组观察存活情况。结果B组4 h2、4 h的血肌酐、尿素氮水平明显高于A组,差异有显著性(p<0.01);A组大鼠肾脏未见明显病理改变,而B组呈典型的IRI改变;C组大鼠均健康存活2周,无感染发生。结论该大鼠肾脏IRI模型的建立操作简便,手术难度低、成功率高,稳定性良好,是研究肾移植IRI的理想动物模型。
Objective To establish a model of kidney ischemia-reperfusion injury（IRI） in rat with auto-orthotopic kidney transplantation, and to observe its stability. Methods Male Sprague-Dawley rats （n = 30） were divided into 3 groups： group A, sham operated group （n-- 10）, group B, auto-orthotopic kidney transplantation group （n = 10） and group C, observation on the stability group （n = 10）. Group A： The right kidney pedicle was ligated at 4 h after low-temperature preservation of the left kidney without occluding pedicle and perfusion. Group B： the left kidney pedicle was occluded, and the left kidney was perfused with low-temperature （ 1℃ to 4℃ ） sodium lactate Ringer＇ s solution by puncturing the left kidney artery, until it turned pale and the outflow from the left kidney vein became clear. Then the kidney artery and vein were repaired with atraumatic suture, The perfused kidney was preserved with the low-temperature preservation system for 4 hours, then reperfused after ligation of the right kidney pedicle. Blood and kidney sample were collected in group A and group B. In group C, the viability was observed, Results Group B had significantly higher serum ereatinine and BUN at 4 and 24 hours than that of group A （p 〈 0.01 ）, Typical pathological changes of IRI were observed in group B, and no obvious changes in group A. All rats in group C had a healthy survival time over 14 days, without infection. Conclusion The model rats have advantages of mild operative difficulty, better stability, without the interference of graft rejection, and are more closely and successfully to simulate the process of lowtemperature preservation in clinical kidney transplantation. Therefore it is an ideal model for IRI studies in kidney transplantation.
出处
《中国比较医学杂志》
CAS
2006年第2期100-103,共4页
Chinese Journal of Comparative Medicine
