摘要
目的探讨梅毒螺旋体特异性IgM抗体检测在各个时期梅毒诊断中的意义。方法采用蛋白印迹法(WB)检测IgM、甲苯胺红不加热血清反应素试验(TRUST)、梅毒螺旋体颗粒凝集试验(TPPA)和暗视野检查梅毒螺旋体(TP)4种方法,对21例一期梅毒患者、8例二期梅毒患者、4例潜伏期梅毒患者、3例怀疑先天性梅毒婴儿、7例正规治疗18个月后梅毒患者及5例健康对照者进行检测分析。结果一期梅毒患者组IgM、TRUST、TPPA、TP阳性率分别为100.0%、42.9%、57.1%和66.7%。二期梅毒患者组、潜伏期梅毒、先天性梅毒患者组IgM、TRUST、TPPA阳性率均为100.0%。正规治疗18个月后梅毒患者组IgM、TRUST、TPPA阳性率分别为0、28.6%和100.0%。健康对照组IgM、TRUST、TPPA均为阴性。结论梅毒螺旋体特异性IgM抗体检测灵敏度高、特异性强,适用于未经治疗的各个时期的梅毒诊断。
Objective To evaluate the significance of IgM treponema pallidum specific antibody in the diagnosis of syphilis in various periods. Methods By using Western bloting（WB）, tolulized red unheated serum test（TRUST）, treponema pallidum particle agglutination（TPPA） and treponema pallidum （TP）, we examined and analyzed 21 eases of first-stage syphilis, 8 cases of second-stage syphilis, 4 cases of latent syphilis, 3 cases of new born infants suspected with congenital syphilis, 7 cases of syphilis formally treated for 18 months and 5 cascs of healthy control groups. Results The positive rates of IgM, TRUST, TPPA and TP in first-stage syphilis cases were 100.0%, 42.9%, 57.1% and 66.7% respectively. The positive rates of IgM, TRUST and TPPA in second-stage syphilis, latent syphilis and congenital syphilis were all 100. 0%. The positive rates in syphilis cases received 18 months treatment were 0%（IgM）, 28.6%（TRUST） and 100.0%（TPPA）. The IgM,TRUST and TPPA in control groups were all negative. Conclusion The specific antibody of IgM treponema pallidum has high sensitivity and specificity,and hence can be applied in the diagnosis of syphilis in various periods.
出处
《国际检验医学杂志》
CAS
2006年第2期108-110,共3页
International Journal of Laboratory Medicine
