摘要
选用100g左右同性、同品系SPF级Wistar大鼠作为实验动物。分为4组,每组(10—20)只,分开饲养。分别为:(1)对照组;(2)半乳甘露寡糖组;(3)半乳甘露寡糖+乳酸菌组;(4)壳寡糖组。在第7、14、21、28d每组各处死4只大鼠,取十二指肠和空肠进行固定、切片、染色,通过HE染色来检测各试验组大鼠肠黏膜结构相关指标的变化;通过Gomori氏改良法来检测肠黏膜碱性磷酸酶(AKP)活性的变化。实验结果显示,不同取材时间,各试验组大鼠十二指肠和空肠绒毛长度和黏膜厚度均高于对照组;不同取材时间,各试验组大鼠十二指肠和空肠黏膜单位面积内AKP阳性物所占面积的百分比均显著高于对照组(P<0.05或P<0.01)。由此推断半乳甘露寡糖、壳寡糖和半乳甘露寡糖+乳酸菌均能改善大鼠肠道黏膜结构,增强小肠吸收功能。添加乳酸菌对半乳甘露寡糖有一定的协同作用,但仍存在一些不稳定性。
Ahout 100 g SPF Wistar rats with the same gender and origin were selected as experimental models, and were randomly divided into 4 groups, 10 20 for each：①control group, ②Galacto-manno-oligosaccharides group, ③ Galacto-manno-oligosaccharides＋ Laetobacillus group, ④Oligochitosan group. 4 rats were sacrificed in the 7th, 14th, 21st, 28th day and their duodenums and jejuna were chose to observe the change of the height of villus, the thickness of gut mucosa by the way of H.E staining. Besides, the activity of AKP was tested through the Gomori staining. The statistics showed that the height of villus and the thickness of gut mucosa in the experimental groups were increased compared with the control group in the different stage, and the activity of AKP in the experimental groups turn out to be remarkably increased （P〈0.05或P〈0.01）. Accordingly, it can be concluded that Galacto-manno-oligosaccharides and Oligochitosan and Galacto-manno-oligosaccharides ＋ Lactobacillus can improve the structure of intestine mucosa, increase the ahsorb-ability to nutrients. Besides, there is a synergistic effect on Galacto-manno-oligosaccharides by Lactobacillus treatment, but it shows the instability.
出处
《科学技术与工程》
2006年第2期131-135,共5页
Science Technology and Engineering
基金
中国科学院知识创新工程重要方向项目(KSCX2-SW-323)
北京市自然科学基金(6022012)资助
