摘要
为了使亚硝基(NO-)更易结合于血红素亚铁离子及提高色素稳定性,本研究采用蛋白酶有限酶解猪血红蛋白,酶解液中大分子血红素多肽与亚硝基反应合成亚硝基血红素多肽。试验表明,以选用木瓜蛋白酶效果较好,最佳酶解条件为:底物浓度16%,加酶量4000U/g,水解时间6h;酶解液合成色素最佳参数为:pH5.00,反应时间12min,反应温度为55℃,NaNO2的添加量为1.6%。
In order to facilitate NO^- to combine with the heme iron ion so as to improve the stability of pigment, this experiment utilized protease to hydrolyze Hb to some degree and then made NO^- react with Heme-iron to concatenate large molecule polypeptide, into nitrosyl heme-potypeptide. The results showed that the optimum enzymatic conditions were： choosing papain, with substrate concentration 16%, enzyme concentration 4000U/g, hydrolysis time 6h, and the optimum conditions for the enzymatic hydrolysate of porcine blood to synthesize nitrosyl heme-polypeptide were： NaNO2 added 1.6%, pH5.00 and reaction time to 12min at 55℃.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第2期131-135,共5页
Food Science
基金
国家自然科学基金项目(30270976)
关键词
猪血红蛋白
蛋白酶
酶解
亚硝基血红素多肽
参数
porcine hemoglobin
protease
enzymatic hydrolysis
nitrosyl heme-polypeptide
parameter
