摘要
根据猪链球菌16 S rDNA基因的种特异性基因序列和CPS基因的2型(1或/和2型)型特异基因序列设计了4条引物,建立了快速检测猪链球菌2型(1或/和2型)的多重PCR方法。利用保存的猪链球菌2型菌株和其他相关标准菌株作为参考菌株对该方法进行了敏感性和特异性试验。结果显示,所建立的多重PCR方法特异、敏感,对组织中的猪链球菌2型(1或/和2型)的最低检出水平为30 CFU/mL。用所建立的多重PCR方法对采自江西、北京、珠海、天津、四川等省市的猪扁桃体样品进行了检测,并通过细菌分离和玻片凝集试验对其检测结果进行了验证,结果显示,检出阳性符合率为100%,证实建立的多重PCR方法可用于猪链球菌2型的快速检测。
Four specific primers were designed based on the species-specific 16 S rDNA gene sequence and type-specific capsular genes sequence of S. suis serotype 2（or 1/2 type）, and then a multiplex PCR assay was established for the rapid detection of S. suis serotype 2（or 1/2 type）. The sensitivity and the specificity tests using S. suis serotype 2 strain and other related standard strains as reference strains showed that the method was quite specific and sensitive, and as low as 30 CPU/mL of serotype 2（or 1/2 type） of S. suis could be detected from tissue samples. The method was also used to detect tonsil samples of pigs from Jiangxi, Beijing, Zhuhai, Tianjin and Sichuan, and the results was consistent with that of classic bacteria isolation and slide agglutination test. The results suggested that the established multiplex PCR assay could be used for rapid detection of Streptococcus suis serotype 2.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2006年第2期112-117,共6页
Chinese Veterinary Science
基金
国家质量监督检验检疫总局应急专项([2005]846)
关键词
猪链球菌2型
多重PCR
检测
初步应用
Streptococcus suis serotype 2
multiplex PCR assay
detection
application
