摘要
目的:观察高乌甲素对甲醛溶液致痛大鼠脊髓背角c-fos基因表达的影响,探讨其脊髓水平的镇痛作用。方法:实验于2003-07/09在潍坊医学院麻醉学系实验室完成。SD大鼠15只,随机分为对照组、单纯甲醛组、高乌甲素组,每组5只。对照组、单纯甲醛组腹腔注射生理盐水,高乌甲素组腹腔注射6mg/kg高乌甲素,2min后单纯甲醛组、高乌甲素组向大鼠右侧后肢跖部皮下注射甲醛溶液,观察动物行为学变化。免疫组化法染色观察脊髓内c-fos基因表达。结果:15只大鼠均进入结果分析。①单纯甲醛组、高乌甲素组注射甲醛溶液后出现缩腿、舔咬注射爪的疼痛反应,注射后马上开始持续约5min的急性早反应(第1期),经过5～10min的静息期,随后出现持续约40min的迟反应(第2期);高乌甲素组在第1期无疼痛反应;第2期时间短于单纯甲醛组[(73±7),(892±72)s,P<0.01]。单纯甲醛组注射处肿胀,直径达2.0～3.0cm,高乌甲素组直径0.5～1.0cm。对照组无异常行为表现。②单纯甲醛组、高乌甲素组见大量淡染Fos样免疫阳性神经元,主要分布在Ⅰ～Ⅱ层及Ⅴ～Ⅶ层。单纯甲醛组及高乌甲素组同侧脊髓腰膨大背角浅层Fos样免疫阳性神经元数明显多于对照组(P<0.01);高乌甲素组同侧背角浅层Fos样免疫阳性神经元数明显少于单纯甲醛组(P<0.01)。结论:高乌甲素处理能对疼痛引起的脊髓灰质背角Fos样免疫阳性神经元增多效应产生抑制作用;脊髓灰质背角Ⅰ～Ⅱ及Ⅴ～Ⅶ层可能是高乌甲素镇痛作用的靶位。
AIM： To observe the the effect of lappaconitine on the genetic expression of formaldehyde solution-induced pain c-fos of cornu dorsale medullae spinalis in rats, and probe into its analgesic effect of spinal cord.
METHODS： The experiment was performed in the Laboratory of Department of Anaesthesiology., Weifang Medical College from July to September 2003. Fifteen SD rats were randomly divided into control group （group C）, simple formaldehyde group （group F） and lappaconitine group （group L） with 5 rats in each group. Rats in group C and F received intraperitoneal injection of normal saline, and rats in group L received intraperitoneal injection of lappaconitine （6 mg/kg）. 2 minutes after that, rats in group F and L were subcutaneously injected of formaldehyde solution into the plantar of right hind limbs, animal ethologys was observed and c-fos genetic expression in spinal cord was observed with immunohistochemical neurocyte staining method.
RESULTS： A total of 15 rats were involved in the analysis of results. ① There were pain reactions in rats of group F and L such as limbcontraction, licking or biting injected claws. Immediately after injection there were acute early reaction, which lasted 5 minutes （first period）, after 5-10 minutes there were resting period, and then delaying reaction lasted 40 minutes （second period）; There were no pain reactions in rats of group L in the first period; While the time in the second period was shorter than that of group F [（73±7）, （892±72） s,P 〈 0.01]. The injected points in rats in group F swelled, the diameter of which was about 2.0-3.0 cm, while that in group L was 0.5-1.0 cm. There were no aberrant behavior or performance in group C. ② There were a great amount of light-stainned and Fos-like immunoreactive positive neurons in group F and L, which mainly distributed in Ⅰ -Ⅱ and Ⅴ-Ⅶ layers. The superficial layer Fos- like immunoreactive positive neurons in ipsilateral spinal intumescentia lumbalis dorsalhorn of group F and L were significantly more than that of group F （P 〈 0.01）; The Fos-like immunoreactive positive neurons in the superficial layer of ipsilateral dorsalhorn were significantly less than that in group F （P 〈 0.01）.
CONCLUSION： Treatment with lappaconitine can inhibit the increasing of Fos-like imnmnoreactive positive neurons in gray nucleus dorsalhorn induced by pain; The gray nucleus dorsalhorn of Ⅰ -Ⅱ and Ⅴ-Ⅶ layers may be the target sites of analgesic effect with lappaconitine.
出处
《中国临床康复》
CSCD
北大核心
2006年第8期82-84,共3页
Chinese Journal of Clinical Rehabilitation
