摘要
目的:探讨戊地昔布对人食管癌Eca109细胞系凋亡及环氧化酶-2表达的影响。方法:采用MTT法检测戊地昔布对人食管癌Eca109细胞生长的作用,流式细胞术检测细胞凋亡和细胞周期分布,电子显微镜进一步检测细胞凋亡,RT-PCR及流式细胞术检测COX-2mRNA及蛋白的表达。结果:戊地昔布(25～400μmol/L)依时间和浓度依赖性抑制人食管癌Eca109细胞的生长,作用72h后,对细胞生长的抑制率可达85.95%,凋亡率由(2.38±0.42)%增加到(48.46±0.73)%;50～400μmol/L时增殖指数和S期的细胞比例则明显降低,G0/G1期的细胞比例增加;同时,低浓度的戊地昔布对COX-2mRNA及蛋白的表达均没有影响,随着浓度的增加明显抑制COX-2mRNA及蛋白的表达。结论:戊地昔布可诱导人食管癌Eca109细胞发生凋亡,其诱导凋亡的机制可能与抑制COX-2的表达有关。
Objective： To investigate the effect of valdecoxib, a selective COX-2 inhibitor, on apoptosis and expression of the COX-2 of human Ecal09 cell line. Methods： MTT assay and flow cytometry were used to observe the effect of valdecoxib on proliferation, apoptosis and the cell cycle distribution of Ecal09 cells. Transmission electron microscope was used to further detect the apoptosis. RT-PCR was used to detect the mRNA expression of COX-2; the expression of COX-2 protein was detected by flow cytometry. Results： Valdecoxib in 25-400（mol. L^-1 significantly inhibited the proliferation of Ecal09 cell line in a time-and dose-dependent way, the inhibition rate of proliferation was 23.09 - 85.95% after 72h, and the rate of apoptosis was increased from （2.38±0.42）% to （2.95±0.83）% （48.46±0.73）%, 50-400mol. L^-1 valdecoxib also decreased the proliferation index and the proportion of cells in the S phase, increased the proportion of cells in the G0/G1 phase , but had no effect on the proportion of cells in the G2/M phase. Compared with those in Ecal09 cells cultured in the medium with solvent, both mRNA and protein level was decreased in the Eca109 cells exposed to valdecoxib in a dose-dependent way in 72h. Conclusion： Valdecoxib could effectively induce apoptosis in the Eca109 cell line, which may be related to inhibiting the expression of COX-2.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2006年第4期213-217,共5页
Chinese Journal of Clinical Oncology
基金
河北省自然科学基金项目资助(编号:301354)
