摘要
目的:研究局部照射He-Ne激光后,兔实验性牙移动牙周组织中转化生长因子β1(transforminggrowthfactorbeta,TGF-β1)表达的变化,探讨弱激光对正畸牙周组织改建的影响。方法:35只健康日本大耳白兔,随机分为7组:未加力组及加力1、3、5、7、14、21d组,每组5只。于双侧上颌第一磨牙至切牙间拴结不锈钢螺簧,80g力拉上颌第一磨牙向近中移动。加力组动物采用自身对照,右侧为照射侧,左侧为对照侧。除1、3d组分别照射1次、3次外,其余各组连续照射5次,每天1次。制作以上颌第一磨牙为中心的近远中方向的组织切片,行TGF-β1免疫组化染色。镜下观察,并对图像分析的灰度积分应用统计学软件SPSS10.0进行两样本均数比较t检验。结果:未加力组与加力各组牙周组织的张力区和压力区中TGF-β1均有表达。压力区照射侧1d组牙周组织TGF-β1表达显著低于对照侧(P<0.05),而3～5d组照射侧TGF-β1表达则显著高于对照侧(P<0.05),高峰值出现在牙受力后第5天。张力区TGF-β1表达在加力3～7d组照射侧高于对照侧(P<0.05),高峰值也出现在牙受力后第5天。结论:He-Ne激光照射有效地促进了TGF-β1在兔实验性牙移动牙周组织中的表达。
PURPOSE： The purpose of this study is to investigate the effect of He-Ne laser irradiation on the expression of transforming growth factor β1 （TGF-β1） during experimental tooth movement in rabbits. METHODS： Thirty-five rabbits were used in this study. The animals were randomly divided into 7 groups equally： normal group and experimental （1, 3, 5, 7, 14, 21days） groups, 5 rabbits in each group. An orthodontic appliance, consisting of a coil spring was ligated to the bilateral first maxillary molar and connected to an orthodontic wire ligated onto the incisors, and exerting a force of approximately 80 g. The left side was used as control, and the right side was designed as irradiated side. The animals from each group were sacrificed at the time discontinued. The histological sections were proceeded with immunohistochemical staining of TGF-β1.Then it was analyzed by Computer Image Analyzing System and statistically processed with SPSS10.0 software package for the paired Student＇s t test. RESULTS： The expression of TGF-β1 was demonstrated in the area of tension and pressure of periodontium tissue in both of the irradiated and control sides. The TGF-β1 staining in the pressure area of the irradiated side decreased significantly at 1 day （P〈0.05） compared with the control side. TGF-β1 staining increased significantly at 3 to 5 days in the pressure area （P〈0.05）. But in the tension area of the irradiated side, TGF-β1 staining were significantly increased at 3 to 7 days （P〈0.05）.The peak value of the area of tension and pressure both appeared at the same time of the 5th day. CONCLUSION： It is concluded that He-Ne laser irradiation can effectively accelerate the expression of TGF-β1 in periodontal tissue of rabbits during experimental tooth movement.
出处
《上海口腔医学》
CAS
CSCD
2006年第1期52-57,共6页
Shanghai Journal of Stomatology
基金
吉林大学创新基金(2002085)
