摘要
目前红细胞临床保存方法主要有低温(4℃)和深低温(-80℃或-196℃)保存。4℃保存时间短,而且容易受到细菌污染;深低温保存大大延长了红细胞保存时间,但需要笨重的低温设备,而且由于保护液中含有甘油等渗透性保护剂,解冻后需要反复洗涤。这些缺陷限制了常规红细胞保存方法在一些特殊情况,例如在战争和自然灾害条件下的应用。相对于常规保存方法而言,冰冻干燥具有以下优势:重量大大减轻,便于运输,适合室温保存,易于再水化。本文就冰冻干燥保存红细胞研究的进展和所面临的挑战,尤其是最近海藻糖在冰冻干燥保存过程中的应用以及其作用机制进行了综合讨论,从而为发展一种安全、简单和有效的红细胞冰冻干燥保存方法提出理论指导。
Now the clinical preservation methods of human red blood cells mainly include hypothermic storage （4℃） and cryopreservation （ -80℃ or - 196℃ ）. The preservation time of hypothermic storage of red blood cells is relatively short and it is easy to be contaminated by microbes. Cryopreservation greatly prolongs the storage time, but it needs heavy storage equipments. Because the protective solutions in cryopreservation contain glycerol, red blood cells need complicated washing in order to remove glycerol. These shortage methods limit their application to some special conditions, such as war or natural disasters. Compared with conventional preservation methods of red blood cells, lyophilization has many advantages such as less weight, convenient transportation, room temperature preservation, prone to be rehydrated. In this review, the progress and challenge in the development of lyophilization of red blood cells, especially application of trehalose and its mechanism in the lyophilization of red blood cells were systematically discussed. This review can provide some theoretic guidance for developing a safe, simple and efficient preservation approach of red blood cells by lyophilization.
出处
《中国实验血液学杂志》
CAS
CSCD
2006年第1期191-196,共6页
Journal of Experimental Hematology
基金
国家自然科学基金资助项目
编号N30400518
关键词
红细胞
冰冻干燥
海藻糖
生物膜
red blood cell
lyophilization
trehalose
biomembrane
