摘要
将不同胚龄的白及种子接种到不同培养基中进行培养,对白及种子萌发率、萌发时间、丛生芽增殖、生根等方面进行了研究。结果表明:白及种子萌发率与其胚龄及有胚率成正相关,萌发时间则与胚龄及有胚率成负相关;胚龄16周的种子在1 g/L花宝1号+2 g/L花宝2号的培养基上萌发率可达84%;胚龄等于或大于20周的种子萌发率不受培养基成分的影响,均可达100%,萌发时间只需7 d;丛生芽增殖的最佳培养基为1/2 MS+4.0 mg/L6-BA+0.2 mg/L NAA+100 g/L CM,其增殖倍数达4.41倍;诱导生根较好的培养基为1/2 MS+0.2 mg/L NAA,生根率达90%。
The effects of different culture medium on the germination rate and time of Bletilla striata Rchb. f. （Orchidaceae） seeds in different developmental phases, and multiplication of cluster shoots and footage of plantlets was investigated. The results showed that the germination rate was positively correlated with the embryo age and the embryo rate of seeds, whereas the germination time was passively correlated with them. For the seeds with 16-week embryo age, the germination rate could be reach 84% on the medium added with 1 g/L Huabao-1 and 2 g/L Huabao-2. The germination rate of the seeds with embryo age more than 20 weeks wasn＇ t affected by the culture medium element and could reach 100%. Moreover, its germination time only need 7 days. The best culture medium for multiplication of cluster shoots is 1/2 MS ＋4.0 mg/L 6-BA ＋0.2 mg/L NAA ＋ 100 g/L CM（ coconut milk）, on which the multiplication rate was 4.41. The medium with 1/2 MS ＋0.2 mg/L NAA was favorable to the growth of roots and the rate of rootage was 90%.
出处
《武汉植物学研究》
CSCD
北大核心
2006年第1期80-82,共3页
Journal of Wuhan Botanical Research
基金
国家863计划项目(2002A241121)资助
关键词
白及
种子萌发
丛生芽
快速繁殖
Bletilla striata Rchb. f.
Seed germination
Cluster shoots
Rapid propagation
