摘要
目的探索支气管哮喘儿童细胞免疫状况的变化。方法采用流式细胞仪检测支气管哮喘儿童急性期及正常对照组外周血T辅助淋巴细胞1(Th1)和Th2的百分率、CD4+、CD3、CD8、NK细胞、B细胞百分率及CD4/CD8比值。对10例哮喘缓解期患儿复查淋巴细胞亚群。结果支气管哮喘患儿发作期外周血Th1细胞百分率[(16.28±9.83)%]明显低于正常对照组[(20.77±6.89)%](P<0.05);哮喘组Th2细胞百分率[(5.44±1.96)%]较对照组[(4.21±2.12)%]明显增高(P<0.05)。Th1/Th2比值,哮喘组为3.41±2.56,对照组为5.91±3.24,两组比较有非常显著性差异(P<0.01)。但哮喘组B细胞、NK细胞百分率、CD3、CD4、CD8及CD4/CD8与正常对照组比较均无统计学差异(P均>0.05)。10例哮喘缓解期患儿淋巴细胞亚群结果表明,B细胞百分率显著下降(P<0.05),其他各项指标在发作期与缓解期无明显差异。结论支气管哮喘儿童最重要的免疫异常是Th1/Th2细胞比例和功能失衡,主要表现为Th2细胞应答优势存在,Th1/Th2显著降低。
Objective To mvestigate the alteration of cellular immunity in asthmatic children. Merits The expressions of T assisrant lymphocyte subsets in peripheral blood were detected in children with asthma compared with normal children using flow cytoraeter, including the percentages of Th1, Th2, CD^4± cells, NK cell, B cell, CD3, CD8 cells, and the ratio of CLM/CDS. Results The percentage of Th1[ （ 16.28 ± 9.83 ） % ] in asthmatic children was significantly lower than that of normal children [ （ 20.77 ± 6, 89） % ] （ P 〈 0.05 ）. The percentage of Th2 [ （5.44 ± 1.96） % ] in asthmatic childran was significantly greater than that of nomual children[ （4.21 ± 2.12） % ] （ P 〈 0.05）. The ratio of Th1/Th2 with asthma was 3.41 ± 2.56, but control group was 5.91 ± 3.24, there was significant difference between two groups（P〈0.01）.There was no difference between asthma and normal children in CD3,CD4,CD8 cells, percentage of NK cell,B cell and the ratio of CD4/CDS（ P 〉0.05）. The T lymphocyte subgroup of 10 children with asthma in the stage of remigsion showed that the percentage of B cell was significantly lower than that of the acute stage of asthma （P〈0.05） , and others had no difference between the two stages of asthma. Conelusion The disorders of the ratio of Th1/Th2 and their functions in asthmatic children are very important, mainly displaying enhancing of the cellular immunity reaction of Th2 and the ratio of Th1/Th2 is significantly lower.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第4期217-218,共2页
Journal of Applied Clinical Pediatrics
