摘要
目的：建立敌敌畏在蔬菜中的残留量的反相高效液相色谱（RP-HPLC）分析方法。方法：用Hypersil C18柱（120A，5μm，4．6mm×300mm）色谱柱，以甲醇-水为流动相，流速1．0ml／min，215nm检测。结果：敌敌畏在0．13～12．0mg／L范围内呈良好的线性关系，检出限为0．113mg／L，添加平均回收率为89．9％，RSD为1．87％。结论：RP-HPLC法简单、可靠，可应用于蔬菜中敌敌畏的残留量的测定。
Objective： To establish a method for the determination of the residues of DDVP in vegetables. Methods： Reversed-phase high performance liquid chromatography was adopted. Hypersil C18 column （5μm, 4.6 mm × 300 mm ID）was used with CH3OH： H2O （65： 35,V/ V ） solution as mobile phase, and the flow speed was 1.0 ml/min. The ultraviolet detection wavelength was 215 nm. Results： Good linear correlation for DDVP was found in the concentration range of 0.13 - 12.0 g/L. The detection limit was 0.113 g/L for DDVP （ S/N = 3 ）. The average recovery rate was 89.9 %, with RSD 1.87 % （n = 5） . Conclusions： This method is simple and reliable. It can be used for the determination of DDVP residues in vegetables with satisfactory results .
出处
《贵阳医学院学报》
CAS
2006年第1期58-60,共3页
Journal of Guiyang Medical College
基金
重庆市自然科学基金资助(CSTC-2004BB7087)
关键词
色谱法
高压液相
敌敌畏
蔬菜
反相高效液相色谱法
chromatography, high pressure liquid
dichlorovos
vegetables
reversed-phase high performance liquid chromatography
