摘要
目的研究氟对原代培养大鼠海马神经元氧化应激和DNA损伤的影响。方法SD大鼠原代培养的海马神经元暴露于20、40、80μg/ml氟化钠24h后,检测海马神经元超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力,还原型谷胱甘肽(GSH)、丙二醛(MDA)的含量,细胞外乳酸脱氢酶(LDH)活力以及DNA损伤的情况。结果40、80μg/ml染毒组海马神经元内MDA含量高于对照组(P<0.05),20、40、80μg/ml染毒组GSH含量、GSH-Px活力低于对照组(P<0.05),80μg/ml染毒组SOD活力低于对照组(P<0.05)。40、80μg/ml组细胞外LDH活力比对照组高(P<0.05)。彗星Olive尾矩升高(P<0.01),相同剂量染氟组细胞内MDA含量与彗星Olive尾矩存在统计学的正相关关系(r=0.895,P<0.05)。结论氟可引起大鼠海马神经元氧化应激和DNA损伤,氧化应激可能在氟致DNA损伤中起重要作用。
Objective To explore the effects of fluoride on oxidative stress and DNA damage in primary hippocampal neurons of rats. Methods The activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px）, the contents of malondialdehyde （MDA） and glutathione （GSH）, the activity of extracellular lactate dehydrogenase （LDH） and DNA damage were detected after the cells were incubated with sodium fluoride at the concentrations of 20,40,80 μg/ml respectively for 24 hours. Results Compared with the control group, the activities of SOD and GSH-Px and the content of GSH were significantly decreased in fluoride-treated groups （P〈0.05）. The content of MDA, activity of LDH and Olive tail moment in fluoride-treated groups were remarkably elevated （P〈0.05, P〈0.05, P〈0.01, respectively）. The significant relationship of positive correlation between MDA content and Olive tail moment was also demonstrated （r=0.895, P〈0.05） in fluoride-treated groups. Conclusion Fluoride can induce oxidative stress and DNA damage in the hippocampal neurons of rats. The oxidative stress may play an important role in the DNA damage induced by fluoride.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第2期125-127,共3页
Journal of Environment and Health
基金
国家自然科学基金资助项目(30371250)
关键词
氟
DNA损伤
氧化应激
海马神经元
Fluoride
DNA damage
Oxidative stress
Hippocampal neuron
