摘要
本文采用电化学方法对铬!-谷胱甘肽(GSH)配合物诱导DNA变性进行表征,同时运用原子力显微镜(AFM)对DNA损伤变性过程进行可视化探测。结果表明:在pH=5.6的HAc-NaAc缓冲溶液中,DNA溶液中加入Cr!-GSH配合物后进行循环伏安扫描,+0.20V和0.00V(vsSCE)处出现一对新的氧化还原峰,该氧化还原峰随Cr!-GSH配合物浓度增加峰电流上升。DNA热变性和表面活性剂SDS变性实验进一步证明了该峰为DNA变性后的氧化还原峰,且变性DNA的峰信号在修饰电极上比裸金电极上更为灵敏。电化学动力学表明在30min内配合物诱导DNA变性的程度随时间的上升而增加,并通过AFM观察了配合物作用下DNA断链的过程。
The damages of DNA induced by Cr（VI）-GSH complexes were characterized by electrochemistry, absorption spectra, and AFM imaging. The redox peaks of denatured DNA appeared at potentials of about ＋0.20 V and 0.00 V in HAc-NaAc buffer solution （pH 5.6） when adding Cr（Ⅵ）-GSH complexes to the DNA solution. Moreover, the peaks current increased with the concentration of Cr（Ⅵ）-GSH complexes and incubation time. The results showed that the Cr（Ⅵ）-GSH complexes resulted in the denaturation of DNA. The heating and SDS denaturation further confirmed the DNA denaturation induced by the Cr（Ⅵ）-GSH complexes. AFM imaging was used to illustrate the cleavage process of DNA.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2006年第3期488-493,共6页
Chinese Journal of Inorganic Chemistry
基金
广东省自然科学基金(No.021190)
广州市科技计划项目(No.2003Z3-D2041)资助。
