摘要
目的:检测益智健脑颗粒浓缩液(YCF)对SAMP/8快速老化小鼠模型行为学、海马神经元凋亡率及凋亡基因Bc l-2和Bax表达的影响,探讨益智健脑颗粒浓缩液改善学习记忆能力的部分作用机制。方法:6月龄SAMP/8快速老化小鼠模型随机分为中药组、西药组、老年对照组,4月龄SAMP/8作为青年对照组,各组给予不同药物干预,8周后水迷宫实验观察行为学改变,应用流式细胞技术、逆转录-聚合酶链反应(RT-PCR)方法测定海马神经元凋亡率和Bc l-2和Bax mRNA的表达。结果:与老年对照组相比,中药组逃避潜伏期缩短,跨平台象限次数增多,凋亡率显著降低;Bc l-2 mRNA表达上调,Bax mRNA表达下调,Bc l-2 mRNA/Bax mRNA相对表达量比率增高。结论:中药益智健脑颗粒能促进Bc l-2表达,抑制Bax的表达,维持Bc l-2/Bax的平衡,降低凋亡率,这可能是其改善SAMP/8快速老化小鼠学习记忆能力的部分作用机制。
Objective To investigate the effect of yizhi jiannao granule concentration fluid （YCF） on the behavior, the apoptosis rate of hippocampus neuron and the expression of apoptosis gene Bcl-2, Bax in senescence accelerated mice Senile-Prone/8 （SAMP/8）, and to discuss some mechanism of traditional chinese medicine YCF in improving the capability of learning and memory. Methods Forty 6-month old SAMP/8 mice were randomly divided into the old group, huperzine A（Hup-A） group and YCF group. Ten 4-month old SAMP/8 mice were served as a young control group. Four groups were given dif- ferent drugs for 8 weeks, their behavior changes were observed, and the hippocampus were taken out to examine the apotosis rate by flow cytomeutry （FCM） and the expression of Bcl-2, Bax mRNA by RT-PCR. Results In the YCF group, the escape latency was significantly shortened, the time of swim in the platform quadrant significantly increased, the apoptosis rate of hippocampus neural decreased; the level of Bcl-2 mRNA and the rate of Bcl-2/Bax increased, and the level of Bax mRNA decreased. Con- clusion Yizhi jiannao granule can decrease the neuron apoptosis rate and the Bax level, increase the Bcl-2 level, and modulate the rate of Bcl-2/Bax in SAMP/8 brain, which is probably part of the mechanisms of inhibiting the apoptosis and improving learning and memory.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2006年第1期56-59,共4页
Journal of Central South University ：Medical Science
关键词
益智健脑颗粒
老年性痴呆
行为学
凋亡
yizhi jiannao granule
the Alzheimer＇s disease
behavior
cell apoptosis
