摘要
在透明介质的表面,利用胶体金单层和蛋白质修饰胶体金的表面等离子体共振放大效应,发展了一种检测生物大分子的新方法,并利用该方法检测了不同浓度的羊抗人 IgG。实验结果表明,该方法不受探针和目标分子体积的影响,不需要昂贵的实验仪器,操作简单,通用性好,并有望制成点阵芯片,实现高通量检测。
This paper presented a new optical method to detect macrobiomolecules on the surface of an optically transparent substrate. The method relied on the enhanced signal of surface plasmon resonance between protein coated colloidal Au and monolayer colloidal Au. Various concentration of goat - anti - huamn IgG were detected and the results showed that this method did not be affected by the size of probe and target molecules, This method is easy, low - cost and has the potential to be constructed as array - chips for high - throughput screening of biomolecules.
出处
《化学传感器》
CAS
2005年第4期33-36,共4页
Chemical Sensors
关键词
表面等离子体共振
胶体金
羊抗人IgG
surface plasmon resonance
colloidal Au
goat - anti - huamn IgG
