摘要
目的观察不同吸烟量的大鼠脑血管内皮细胞基质金属蛋白酶9的表达,初步探讨吸烟导致脑缺血的发病机制。方法选择健康Wistar大鼠60只,分为正常对照组、短期大量吸烟组、长期大量吸烟组、长期小量吸烟组、戒烟组和非吸烟组。通过免疫组织化学染色观察大鼠脑血管内皮细胞基质金属蛋白酶9的表达。结果基质金属蛋白酶9阳性反应物呈棕黄色,正常对照组及非吸烟组细胞基质金属蛋白酶9表达极少,余各组有不同程度表达,且与对照组比较有显著性差异(P<0.05)。结论吸烟可致脑血管内皮细胞基质金属蛋白酶9表达增加,且随着吸烟量和吸烟时间的增加表达增多,戒烟后表达下降。细胞基质金属蛋白酶9在吸烟时脑血管内皮细胞的表达增加可能是吸烟致脑梗死的主要途径之一。
Aim The ischemic cerebrovascular disease （ ICVD） has been commonly thought one of the three main causes of death in human. The dangerous factors include hypertension, diabetes, atherosclerosis, smoking, drinking and so on. Smoking has been confirmed independent factor in stroke. Matrix metalloproteinase is one of main enzemy dissolved extral cell membrane. Studies have indicated that matrix metalloproteinase-9 had played an important role in the arteriosclerosis. They all join the appearance of the cerebral infarction. Methods Materials 60 healthy Wistar rats supplied by Department of experiment animal of China Medical University were used. Monocolone antibody of MMP-9 and SABC test box were from BOSTER Biotechnology CO. LTD. Cigarettes were purchased from markets. Method The Wistar rats were divided into 6 groups： normal group （ n = 6）, short-time and big-dose smoking group （ n = 12）, long-time and big-dose smoking group （ n = 12}, long-time and small-dose smoking group （ n = 12）, giving-up smoking group （ n = 12）, control group （ n = 6）. We obtained brain following experiment ending and observed expression of smoked rat by immunohistocbemistry stain. Results The positive substance of MMP-9 showed brown. The expression of MMP-9 in normal group and control group is weak. The significant different expression is observed in other groups （P 〈 0.05 ）. Giving up smoking can reduce the expression of MMP-9. Conclusions Smoking can make MMP-9 express more in endothelial cell. The expression of MMP-9 is dese-dependent and fime-dependent. Giving up smoking can reduce expression of MMP-9. The expression of MMP-9 in cerebrovascular endothelial cell may be one of main ways in cerebral infarction caused by smoking.
出处
《中国动脉硬化杂志》
CAS
CSCD
2005年第5期583-585,共3页
Chinese Journal of Arteriosclerosis
