摘要
目的:探讨骨碎补类黄酮提取物(flavonoid fraction,FF)对氯化汞(HgCI2)所致的急性肾衰竭(ARF)大鼠模型的保护作用。方法:利用HgCI2诱导的ARF动物模型,将大鼠随机分成正常对照组、HgCI2(模型组)、HgCI2+FF组、FF组。在实验第24、48、72h检测血生化指标,光镜观察肾组织改变,免疫组化染色检测增殖细胞核抗原(PCNA)、ED-1在肾组织的表达,同时检测肾组织MDA及GSH含量。结果:HgCI2+FF组肾脏病理改变比HgCI2组减轻,同时血肌酐下降(P<0.05);免疫组化显示PCNA、ED-1在肾间质表达下调,均与HgCI2组有统计学差异(分别P<0.01及P<0.05);FF还可预防肾组织因HgCI2毒性所致的MDA含量升高,GSH下降(P<0.05)。结论:FF通过抗氧化作用,减轻HgCI2诱导的急性肾衰竭。
Objective:We investigate the effects of the flavonoid fraction(FF) from Drynaria fortunei on the rats with acute renal failure (ARF) caused by mercuric chloride(HgCl2). Methods:By means of the animal model of ARF induced by HgCl2, BALB/c rats were divided into 4 groups:normal group,model group(HgCl2) ,HgCl2 + FF group and FF group. At the 24th ,48th and 72th hours after the insult, serum creatinine(Scr) , renal pathological changes, PCNA and ED- 1 cells in renal tissues were studied, we also determined the renal content of malondialdehyde(MDA) and glutathione(GSH). Results:Pathological change in HgCl2 + FF group was more slight than that in HgCl2 group, meanwhile, the Scr was reduced in FF treated group (P〈0.05) ;The PCNA and ED- 1 positive cells decreased in the HgCl2 + FF group (P〈0.01 and P〈0.05,respectively) ; Increrment in renal content of MDA and decrease in GSH resulting from HgCI2 toxicity were prevented by FF treatment( P 〈 0.05). Conclusion: FF treatment might ameliorate HgCl2 - induced ARF through its antioxidant function.
出处
《中国中西医结合肾病杂志》
2006年第2期75-79,i0001,共6页
Chinese Journal of Integrated Traditional and Western Nephrology
关键词
急性肾衰竭
骨碎补
氧化应激
氯化汞
Acute renal failure Drynaria fortunei Antioxidant function Mercuric Chloride