摘要
采用包埋脱水法冰冻保存牟氏角刺藻(Chaetocerosmuelleri)、小新月菱形藻(Nitzschiaclosteriumminutissimai),探讨了预培养对两种硅藻存活率的影响.结果表明,用0.6M的蔗糖预培养时,牟氏角刺藻冰冻后的存活率有提高,最大可提高7.2%.但预培养对小新月菱形藻的存活率没有明显提高.
Based on the preservation of the Chaetoceros muelleri and Nitzschia dosterium minutissimai by encapsulation-dehydration, the influences of pre-culture on the two diatoms＇ algal viabilities are discussed in this paper. The experiment shows that the algal viability of Chaetoceros muelleri increases after cryopreservation when it was pre-cuhured with 0.6 M of sucrose,the rate of it can reach 7.2% at most. In term of Nitzschia closterium minutissimai ,no obvious clue to show any influence.
出处
《鞍山师范学院学报》
2005年第6期68-70,共3页
Journal of Anshan Normal University
关键词
牟氏角刺藻
小新月菱形藻
包埋脱水法
冰冻(超低温)保存
预培养
Chaetoceros m uelleri
Nitzschia closterium minutissimal
Encapsulation-dehydration
Cryopreservation
Pre-culture
