摘要
目的观察大鼠脑缺血再灌注后神经细胞巢蛋白(Nestin)、干细胞因子(SCF)和神经细胞黏附分子(NCAM)基因的表达。方法成年健康雌性SD大鼠36只,以线栓法建立大脑中动脉缺血再灌注模型,随机分为缺血1.5 h再灌注2 h、6 h、12 h、24 h、2 d、3 d、7 d、14 d组和假手术组,每组4只。应用原位杂交技术检测脑缺血再灌注后脑组织Nestin、SCF和NCAM mRNA的表达。结果假手术组皮质和纹状体区Nestin、SCF和NCAMmRNA均有微弱表达。脑缺血再灌注后Nestin mRNA表达增高,皮质除再灌注2 h以外、纹状体除再灌注2、6 h以外,其余各时间点均明显高于假手术组。SCF mRNA表达,皮质除再灌注2、6、12 h以外,纹状体除再灌注2、6 h以外,其余各时间点均明显高于假手术组。NCAM mRNA于再灌注2 h后在皮质和纹状体区开始表达,分别于再灌注12 h和1 d达高峰,7 d后逐渐减少,14 d降至假手术组水平。结论脑缺血再灌注后SCF mRNA表达可能具有促进神经干细胞增殖作用,NCAM表达可能参与了损伤后脑组织的修复过程。
Objective To study the gene expressions of Nestin, stem cell factor （SCF） and neural cell ad- hesion molecule （NCAM） in neurons after ischemia-reperfusion injury in rat brain. Methods Thirty-six adult female rats were induced by intraluminal middle cerebral artery （MCA） occlusion, and divided into ischemia-1.5 h group and reperfusion 2 h-, 6 h-, 12 h-, 1 d-, 2 d-, 3 d-, 7 d-, 14 d- groups and sham-operated group （n=4）, with a nylon monofilament suture. In situ hybridization was used to examine the expression of Nestin, SCF and NCAM mRNA. Results There were basic expression of Nestin, SCF and NCAM mRNA in brain tissue of sham-operated group. Nestin mRNA expression in cortex and striatum increased markedly in ischemic hemisphere compared with sham-operated group except reperfusion 2 h in cortex and 2 h, 6 h in striatum. SCF mRNA expression in cortex and striatum increased significantly in ischemic hemisphere compared with sham-operated group except reperfusion 2 h, 6 h, 12 h in cortex and 2 h, 6 h in striatum. NCAM mRNA expression was observed after reperfusion 2 h, peaked at 12 h and 1 d in cortex and striatum, respectively, and decreased to the basic level of sham-operated group at 14 d. Conclusion The results indicate that SCF expression might enhance neural stem cell proliferation following ischemia reperfusion. The increasing NCAM expression might play an important role in the neural repair of injured brain tissues.
出处
《青岛大学医学院学报》
CAS
2006年第1期27-31,共5页
Acta Academiae Medicinae Qingdao Universitatis
基金
FundedbytheNaturalScienceFoundationofShandongProvince(Y2001C04)
