摘要
利用两个小麦-黑麦异源双代换系DS 5A／5R与DS 6A／6R杂交,探讨同祖染色体配对的可能性与创制小麦黑麦异源易能系。在方法上对杂种F1的减数分裂行为进行研究,观察5R与5A、6R与6A配对频率,探讨同祖染色体配对规律。实验结果看到:杂交F1减数分裂中有22．91％的花粉母细胞有小麦染色体(ABD组)与黑麦染色体(R组)发生同祖配对。在F2及以后世代,通过染色体C分带、原位杂交检测,选择小麦-黑麦易位系。在F2代的4株中5检测到9株有易位,易位频率为20％,是目前小麦-黑麦染色体易位频率最高的。染色体易位有的来源于不同祖配对的交换,有的来源于单价体错分裂或断裂的重建。
This article aims to study the homoeologous chromosome pairing and translocation induced by 5A/SR×6A/6R wheat-rye substitution lines. To clarify the mechanism of homoeologous chromosome pairing and create wheat-rye translocation lines, two wheat-rye substitution lines, 5A/5R and 6A/6R were crossed. The chromosome behavior of pollen mother cells （PMCs） in meiosis was investigated in hybrid Fl. Homoeologous chromosome pairing between wheat and rye occurred in 22.91% of PMC. Wheat-rye translocation lines were identified via C-banding and in situ hybridization （GISH） in hybrid F2 and later generations. In F2 generation, translocations happened in 9 of 45 plants, reached to 20%. These translocation lines were generated from homoeologous chromosome pairing, or mis-division and reconstruction of univalent chromosomes.
基金
This work was supported by the National Natural Science Foundation of China (No. 39670417).
关键词
代换系
易位系
同徂配时
原位杂交
homoeologous chromosome pairing
translocation
wheat-rye substitution line
meiosis
GISH
