摘要
目的探讨人毛囊干细胞定位和增殖特性。方法取人枕部头皮毛囊进行角蛋白19(K19)免疫组化染色,并测量阳性段距毛囊下端的距离;将包含毛球部上方阳性段的毛囊上皮组织分别置于含有和不含有间质细胞的凝胶表面气液界面培养,当出现增殖灶时,测量其距毛囊下端的距离,分析K19阳性部位与培养出现的增殖灶之间的关系,并通过透射电镜观察增殖灶的超微结构。结果人枕部头皮毛囊有两个K19阳性区域,即隆突部和毛球部上方一段外根鞘,毛囊下段上皮组织只在含有间质细胞的凝胶表面培养时毛球部上方出现一增殖灶,统计学分析支持毛球部上方的K19阳性部位和毛囊下段上皮组织培养出现的增殖灶为同一部位。透射电镜显示增殖灶含有幼稚细胞、成熟角质形成细胞和凋亡细胞。结论人头皮毛囊可能有两个干细胞库,即隆突部和球部上方外根鞘的一个局限区域,干细胞的增殖需要间质细胞(如毛乳头细胞)的存在,增殖的干细胞可能向形成新的干细胞、成熟角质形成细胞和凋亡细胞三个方向演变。
Objective To investigate the location and proliferation of human hair follicle stem cells. Methods The expression of keratin 19 （K19） in the human hair follicles on the occiput was detected by immunohistochemical staining, and the positive area was located. The follicle epithelium containing the K19-positive area above the hair bulb were cultured with or without mesenchymes （dermal papilla cells, DPCs） at the air-liquid interface of the collagen gel. When the proliferating colonies formed, the distance from the colony to the bottom of hair follicles was measured and the relationship between the K19-positive position and the proliferation colonies was analyzed. The ultrastructure of the proliferation colony was observed by transmission electron microscopy. Results Two portions were found positive for K19 in the outer root sheath of the human hair follicles, the upper one being at the bulge region and the lower one at the outer root sheath above the hair bulb. The proliferating colony formed only when the epithelium of the hair follicle was co-cultured with mesenchymes cells. The statistical analysis suggested the lower portion positive for K19 and the proliferation colonies in the culture were traced to locate at the same site of the human hair follicles. Under electron microscopy, juvenile cells, mature keratinocytes and apoptotic cells were found in the proliferating colonies. Conelusions The human hair follicles may contain two distinct reservoirs for stem cells, which locate in the bulge area and in the region of the outer root sheath above the hair bulb, respectively. Mesenchymes are needed for the proliferation of stem cells. New stem cells, mature keratinocytes and apoptotic cells may be three endings of the stem cells＇ proliferation.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2006年第3期157-160,共4页
Chinese Journal of Dermatology
基金
国家自然科学基金资助课题(30571686)
关键词
毛囊
干细胞
Hair follicle
Stem cells
