摘要
目的探讨肾盂输尿管连接部(UPJ)梗阻对对侧肾的影响。方法采用新西兰大白兔24只,随机分为完全梗阻组(组1)、部分梗阻组(组2)和假手术组(组3),每组8只。组1、2分别行右侧输尿管完全、部分结扎,组3只显露而不结扎输尿管。术后4周处死,切除对侧肾。处死前尿路造影示组1、2兔均发生右侧肾积水。应用电子天平测定对侧肾实质重量,采用FITC-Annexin-V/PI双染色流式细胞仪检测对侧肾皮髓质细胞凋亡率。所有标本均行病理学检查。结果4周后,组1、2、3对侧肾实质重量分别为(3.96±0.20)、(3.47±0.19)、(3.20±0.21)g/kg体重,组1、2与组3比较差异均有统计学意义(P<0.05);对侧肾皮质的细胞凋亡率分别为(2.19±0.83)%、(2.17±0.79)%和(2.14±0.76)%,组间两两比较差异均无统计学意义(P>0.05);对侧肾髓质的细胞凋亡率分别为(23.75±2.34)%、(11.82±1.40)%和(2.36±0.65)%,组间两两比较差异均有统计学意义(P<0.01)。UPJ梗阻4周后,组2对侧肾间质充血、轻度水肿,部分肾小管扩张,管腔内存在坏死脱落的物质;组1对侧肾改变更为明显。结论UPJ梗阻不仅引起对侧肾实质代偿性增生,同时还造成对侧肾损害。
Objective To explore the effects of ureteropelvic junction（UPJ） obstruction on contralateral kidney. Methods Twenty-four adult New Zealand rabbits were divided randomly into 3 groups,ie, complete obstruction group （ group 1, n= 8 ）, partial obstruction group （ group 2, n = 8 ） and shamoperation group （ group 3, n = 8）. In groups 1 and 2, the right ureter was ligated completely or partially. In group 3, the right ureter was only exposed but not ligated. The animals were killed 4 weeks after operation, and the contralateral kidneys were resected. Before killing, anterior urography showed that right hydronephrosis occurred in all the rabbits in groups 1 and 2. The contralateral renal weight was measured by electron balance. The apoptosis in the contralateral renal cortex and medulla was detected by FITC-Annexin-V/PI flow cytometric assay. The morphological changes of the samples were also studied. Results After 4 weeks, the contralateral renal weight of the rabbits in groups 1, 2 and3 was （3.96 ±0.20） ,（3.47±0.19） and （3.20 ±0.21） g/kgBW, respectively. The difference of contralateral renal weight between groups 1, 2 and group 3 was significant （P 〈 0.05）. The apoptosis rates of contralateral renal cortex cells in groups 1, 2 and 3 were （2.19 ± 0.83 ） %, （ 2.17 ± 0.79） % and （2.14 ± 0.76 ） %, respectively. There was no significant difference of the rates between these 3 groups （P 〉 0.05 ）. In contrast, the apoptosis rates of eontralateral renal medulla cells in groups 1, 2and3 were （23.75±2.34）%,（11.82±1.40）% and （2.36±0.65）%, respectively. There were significant differences between these 3 groups （P 〈 0.01 for all）. UPJ obstruction for 4 weeks led to histologic alterations in the contralateral kidney such as interstitial edema,congested blood vessels and dilated tubuli with necrotic deposits in them, which were more prominent in complete obstruction group than in partial obstruction group. Conclusions UPJ obstruction leads not only to parenchymal hypertrophy but also to parenchymal damage in the contralateral kidney.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2006年第3期158-161,共4页
Chinese Journal of Urology
关键词
肾盂输尿管连接部
梗阻
凋亡
Ureteropelvic junction
Obstruction
Apoptosis
