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耐甲氧西林溶血葡萄球菌、金黄色葡萄球菌erm基因的检测与分析 被引量：1

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摘要 目的比较耐甲氧西林溶血葡萄球菌、金黄色葡萄球菌erm基因的检出差异、以及与细菌药敏表型之间的关系。方法用MicroScan auto SCAN4细菌鉴定仪进行鉴定和药敏试验、用聚合物酶链反应(PCR)法检测erm基因。结果36株耐甲氧西林金黄色葡萄球菌(MRSA)中表型耐红霉素的为35株,其中有34株检出erm基因,两者符合率为97.14%;24株耐甲氧西林溶血葡萄球菌(MRSH)中表型耐红霉素的为22株,其中有15株检出erm基因数,两者符合率为68.18%。两组细菌数据统计使用电子表格Microsoft Excel软件处理,组间率比较采用卡方检验,P<0.05,差异非常显著。结论耐甲氧西林金黄色葡萄球菌对红霉素的耐药机制主要是由erm基因编码甲基化酶,使药物作用靶位的改变。耐甲氧西林溶血葡萄球菌对红霉素耐药不仅存在erm耐药基因,还有着其他的耐药分子机制。
作者 王宏 谢忆虹 彭梅 糜祖煌
机构地区 南昌市第一医院 无锡市克隆遗传技术研究所
出处 《江西医学院学报》 2006年第1期111-112,共2页 Acta Academiae Medicinae Jiangxi
关键词 耐甲氧西林 葡萄球菌 耐药 基因
分类号 R446.51 [医药卫生—诊断学]
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  • 1Roberts MC,Sutcliffe J,Courvalin P,et al.Nomenclature for macrolide and macrolide—lincosamide—streptogramin B resistance determinants[J].Antimicrob Agents Chemother,1999,12:2823—2830.
  • 2Hussain Z, Stoakes L, Garrow S, et al. Rapid detection of mecA-positive and mecA-negative coagulase-negative staphylococci by an anti-penicillin binding protein 2a slide latex agglutination test. J Clin Microbiol, 2000,38(6) : 2051.
  • 3Trine G, Claus K, Trond F, et al. Rapid detection of the methicillin-resistance gene, mecA, in coagulase-negative staphylococci. Scand J Infect Dis,2001,33(7) :502.
  • 4De Neeling AJ, van Leeuwen WJ, Schouls LM, et al. Resistance of staphylococci in the Netherlands: surveillance by an electronic network during 1989-- 1995. J Antimicrob Chemother, 1998,41(1) :93.
  • 5Voss A, Kresken M. Antibiotic resistance in staphylococci in Europe. Chemotherapy, 1996,42(suppl 2) : 13.
  • 6Coleman K, Athalye M, Clancey A, et al. Bacterial resistance mechanisms as therapeutics target. J Arltimicrob Chemother, 1994,33(6) : 1091.
  • 7Pascual A, Ramirez de, Arellano E, et al. Activity of glycopeptides in combination with amikacin or rifampin against staphylococcus epidermidis biofilms or plastic catheters. Eur J Clin Microbiol Infect Dis, 1994,13(6) :515.
  • 8Srinivasan A, Dick JD, Perl, TM. Vancomycin resistance in staphylococci. Clin Microbiol Rev,2002,15(3) :430.
  • 9Petinaki E, Kontos F, Miriagou V, et al. Survey of methicillin-resistant coagulase-negative staphylococci in the hospitals of central Greece. Intern J Antimicrob Agents, 2001,18(6) : 563.
  • 10Raimundo O, Heussller H, Bruhn JB, et al. Molecular epidemiology of coagulase-negative staphylococcal bacteraemia in a newborn intensive care unit. J Hosp Infect, 2002,51(1) : 33.

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  • 1吴永乐,张永信.金葡菌对喹诺酮类药物的耐药机制[J].国外医药（抗生素分册）,1996,17(3):216-219. 被引量：12
  • 2Katayama, Y. , Ito, T. , Hiramatsu, K. A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus[ J]. Antimicrob Agents Chemother, 2000, 44(6) : 1 549-1 555.
  • 3Vesterholm-Nielsen, M. , Olhom Larsen, M. , Elmerdahl Olsen, J. , et al. Occurrence of the blaZ gene in penicillin resistant Staphylococcus aureus isolated from bovine mastitis in Denmark[J]. Acta Vet Scand, 1999, 40(3) : 279-286.
  • 4Ubukata, K. , Nonoguchi, R. , Song, M. D. , et al. Homology of mecA gene in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans to that of Staphylococcus aureus [J]. Antimicrob Agents Chemother, 1990, 34(1) : 170-172.
  • 5Stratidis, J. , Bia, F.J. , Edberg, S. C. Use of real-time polymerase chain reaction for identification of methicillin-resistant Staphylococcus aurcus directly from positive blood culture bottles [J]. Diagn Microbiol Infect Dis. , 2007, 58 (2) : 199-202.
  • 6Anderson, M.E. , Weese, J. S. Evaluation of a real-time polymerase Chain reaction assay for rapid identification of methicillin-resistant Staphylococcus aurcus directly from nasal swabs in horses[J]. Vet Microbiol, 2007, 122(1-2): 185-189.
  • 7Berger-Bachi, B. , Barheris-Maino, L. , Strassle, A. , et al. FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: molecular cloning and characterization[ J]. Mol Gen Genet, 1989, 219 ( 1-2 ) : 263-269.
  • 8Sabet, N.S. , Subramaniam, G. , Navaratnam, P. , et al. Detection of mecA and ermA genes and simultaneous identification of Staphylococcus aureus using triplex real-time PCR from Malaysian S. aureus strain collections [ J ]. Int J Antimicrob Agents, 2007, 29 (5) : 582-585.
  • 9Chang, S. , Sievert, D. M. , Hageman, J. C. , et al. Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene[J]. N Engl J Med., 2003, 348( 14): 1 342-1 347.
  • 10Sinsimer, D. , Leekha, S. , Park, S. , et al. Use of a multiplex molecular beacon platform for rapid detection of methicillin and vancomycin resistance in Staphylococcus aureus[J]. J Clin Microbiol, 2005, 43(9) : 4 585-4 591.

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江西医学院学报
2006年 第1期

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