摘要
目的:观察正常大鼠气道TLR4的分布,LPS对大鼠气道TLR4分布以及蓝桉油对TLR4表达的影响。方法:气管内注入LPS复制大鼠急性肺损伤模型,观察72 h后的病理变化、支气管肺泡灌洗液中细胞总数的变化、免疫组织化学特点以及蓝桉油的影响。结果:免疫组化结果显示TLR4在正常组大鼠气道内有广泛分布。急性肺损伤组大鼠支气管肺泡灌洗液细胞总数明显增高,病理结果显示支气管、肺内炎症程度均明显高于正常组,免疫组化结果显示主支气管和肺内细支气管上皮细胞内TLR4表达明显增强。蓝桉油300 mg.kg-1组支气管肺泡灌洗液中白细胞总数明显低于急性肺损伤组,病理结果显示气管和主支气管的炎症程度明显减轻,免疫组化提示肺内细支气管上皮细胞内TLR4的表达量减少。结论:TLR4在大鼠气道内广泛分布,LPS刺激可使TLR4的表达增强,蓝桉油可降低由LPS引起的肺内细支气管上皮细胞内TLR4表达的增高。
Objective： To observe the distribution of toll-like receptor 4 （TLR4） in rats＇ respiratory tract. To study the influence of IPS and Eucalyptus globulus oil on the distribution of TLR4. Method： The Sprague-Dawley rats were intratracheally instilled with lipopolysaccharide （LPS, 2 mg·kg^-1 per day） for two days to induce acute lung injury. The rats were sacrificed at 72 hours after IPS instillation. Lung morphology was studied. Leukocytes in Bronchoalveolar lavage fluid （BALF） were measured and TLR4 were detected by immunohistochemistry. Result： The result of immunohistochemistry showed that TLR4 distributed widely in common rats＇ respiratory tract. In the group of acute lung injury, the number of leucocyte in BALF was increased apparently, the inflammation in bronchus and bronchioles was more apparently than that of the control group in morphology. And the expression of TLR4 was reinforced in main bronchus and broochioles. In the group of E. globules oil （300 mg·kg^-1）, the leucocyte number was decreased apparently in BALF, the inflammation was lightened and the exxon of TLR4 decreased as compared with the group of models. Conclusion： The expression of TLR4 distributes widely in rats＇ respinatory tract. The stimulation of LPS can reinforce the expression of TLR4. The E. globules oil can reduce the increase of TLR4 induced by LPS in bronchioles.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第4期319-322,共4页
China Journal of Chinese Materia Medica
基金
浙江省中医药管理局重点科研项目(kf0007)
关键词
脂多糖
TLR4
蓝桉油
急性肺损伤
lipopolysaccharide
toll-like receptor 4
Eucalyptus globulus oil
acute lung injury
