摘要
目的探讨络通对不同病程的糖尿病大鼠视网膜微血管形态学改变的防治作用。方法采用链脲佐菌素(65㎎/㎏)经大鼠腹腔一次性注射制备糖尿病模型,成模糖尿病大鼠随机分成糖尿病模型组、成模后即开始络通干预6个月组(络通干预组)和成模6个月后开始络通治疗3、6个月组(络通治疗1、2组)。分别按期摘取眼球,做普通病理及透射电镜观察,并利用LEICA-Q550IW计算机多功能真彩色病理图像分析系统定量分析视网膜微血管基底膜厚度。结果与6个月模型组比较,光镜和电镜观察下络通干预组视网膜微血管形态学改变明显减轻。9个月时,模型组大鼠视网膜微血管改变进一步加重;络通治疗3个月后,视网膜微血管改变较9个月模型组有所减轻,但病变仍在进行性加重。12个月时,模型组大鼠视网膜微血管形态学改变非常严重,络通治疗6个月后,整体微血管改变较12个月模型组明显减轻,与9个月模型组无明显差异。计算机图像分析显示络通干预组及络通治疗1、2组视网膜毛细血管基底膜厚度与同期模型组比较有非常显著性差异(P<0.01)。结论络通对糖尿病大鼠视网膜微血管病变具有较好的防治作用,且早期干预胜于后期治疗。
Objeective To study the protective and therapeutic effect of Luotong on the retinal microvessel kinetic morphological changes during various stages of streptozotocin-induced diabetic rats. Methods The diabetic rat model was setup by a single intraperitoneal injection 65 mg/kg of streptozotocin. Diabetic model rats were randomly divided into diabetic model group （6, 9, 12 months subgroups）, diabetic group protected with Luotong （6 months） from the onset of diabetic model （LT group）and diabetic group treated with Luotong （3, 6 months subgroups ） 6 months after the onset of diabetic model （LT1, 2 group）. The eyeballs were removed at 6, 9 and 12 months, respectively. The whole mounts of retinal digestion were prepared and stained with PAS staining method. The light microscope and transmission electron microscope morphological observations were performed and the basilar membrane thickness of retinal capillaries were quantitatively analysed by LEICA-Q550IW computer image system. Results At 6 months, the retinal microvessel morphological changes of LT group were less severe than those of 6 month model group. At 9 months of the diabetic model rat, the retinal microvessel morphological changes were prominent. 3 months after Luotong therapy, the retinal microvessel changes were partly improved in comparison with 9 month model group but still aggravated gradually. At 12 months, the retinal microvessel morphological changes of diabetic model rats were very severe. 6 months after Luotong therapy, the retinal microvessel changes were significant mitigated compared with 12 month model group. The results of computer image analysis indicated the retinal capillary basilar membrane thickness in diabetic group protected or treated with Luotong were significant decreased in comparison with homeochronous model group （P〈0.01）. Conclusion Intervention and treatment of Luotong maybe be significant helpful in delaying or possibly preventing against the occurring and development of diabetic-like retinopathy in rats. Early intervention is superior to later treatment.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第3期25-27,共3页
Chinese Journal of Information on Traditional Chinese Medicine
