摘要
建立川菊中叶黄素含量的检测方法.采用高效液相色谱法,以叶黄素为指标,D iamonsilTMC18柱(4.6mm×150mm,5μm);CH3OH-H2O(95.5?4.5)为流动相;检测波长为444.5 nm.结果:平均回收率为99.23%,RSD=0.6%.进样量在0.0027-0.0189μg线性关系良好,r=0.9998.该方法简便、稳定、重现性好,为菊花质量的控制提供参考.
The approach is established to determine the xanthophyl contents in chrysanthemum morifolium ramat. The high -proficiency liquid colour is adopted as method, xanthophyl as index and Diamonsil^TMC18 （4.6mm 150mm, 5μm） as column; CH3OH - H2O（95.5：4.5）as mobile phase. The wavelength for determination is 444. 5mm. Results ： The average rate of recovery is 99.23% and RSD is 0.6%. Lineal relation is better when the sample is 0. 0027 -0. 0189μg r=0. 9998. The determination approach which can be used to control the quality of chrysanthemum is convenient, stable and has good recurrence characteristic.
出处
《西南民族大学学报（自然科学版）》
CAS
2006年第1期96-98,共3页
Journal of Southwest Minzu University(Natural Science Edition)
关键词
HPLC
川菊
叶黄素
含量测定
HPLC
chrysanthemum morifolium ramat
xanthophyl
contents determined
