摘要
目的观察慢性乙型肝炎患儿外周血树突状细胞(DC)亚群的变化,探讨DC亚群在乙型肝炎慢性化中的意义。方法慢性乙型肝炎患儿组26例,HBV携带儿童组16例,健康儿童25例,利用流式细胞技术对患者外周血髓样树突状细胞(mDC)和浆细胞样树突状细胞(pDC)的百分比和绝对值进行检测,并用CpG2216刺激外周血单个核细胞(PBMC),检测pDC的α干扰素产生能力。结果与健康对照组比较,慢性乙型肝炎患儿组血mDC和pDC的百分比和数量均显著下降(P<0.05),PBMC经CpG2216刺激后产生干扰素的能力较健康对照组也有显著下降(P<0.05),而HBV携带儿童仅pDC百分比与健康儿童比较有明显下降(P<0.05)。相关性分析表明,HBV携带儿童pDC绝对值与HBV DNA水平之间存在正相关(r=0.5464,P=0.0351),而慢型性乙型肝炎患儿mDC绝对值与HBV DNA水平存在负相关(r=-0.4554,P=0.0194)。结论慢性乙型肝炎患儿外周血DC亚群下降的频率和数量导致其功能损伤,可能与HBV持续感染的致病机制有关。
Objective To investigate the characteristics of the circulating dendritic cell （DC） subsets in HBV-infected children. Methods Twenty six children patients with chronic HBV, eleven asymptomatie HBV can＇iers and twenty five healthy individuals as controls were enrolled in our study. The percentage and absolute numbers of the myeloid （mDCs） and plasmacytoid dendritic cells （pDCs） in blood are detected by flow eytometry. The peripheral blood mononuclear cell （PBMC） was stimulated for 24 h by toll-like receptor 9 ligand, CpG 2216. The supernatants were collected and IFN-αwas determined by ELISA assay. Then, the IFN-α-producing capacity of pDCs was measured. Results Compared with healthy controls, the mDC and pDC percentage and counts were significantly decreased in chronic HBV-infected children. The pDCs＇ IFN-α-releasing capacity was also decreased compared with that of healthy children. In asymptomatic HBV carriers, only the pDC percentage significantly decreased compared with healthy controls. In addition, pDC counts were significantly correlated positively with serum HBV DNA levels in asymptomatie HBV carriers （ r = 0.5464, P = 0. 0351 ）. And mDC counts were significantly correlated negatively with serum HBV DNA levels in chronic HBV-infected children （r = - 0. 4554, P = 0.0194）. Conclusion The decrease of circulating DC subset percentage and counts results in their functional impairment, which may contribute to the persistence of HBV infection.
出处
《肝脏》
2006年第1期1-3,共3页
Chinese Hepatology
关键词
乙型肝炎
儿童
树突状细胞
Hepatitis B virus
Children
Dendritic cells
