摘要
根据副溶血性弧菌的耐热溶血素基因（ｔｄｈ）设计了２对引物，经聚合酶链式反应（ＰＣＲ）检测，所有１８株神奈川试验阳性副溶血性弧菌均呈阳性反应，而６株神奈川试验阴性副溶血性弧菌呈阴性反应，其他９种（３４株）弧菌和８株其他革兰氏阴性菌也呈阴性反应。检测敏感性可达２ＣＦＵ的副溶血性弧菌。
Two sets of primers based on the unique
regions of the tdh geiie of V.parahaemolyticus were designed.A specificity test dernonstrated
that the polymerasechain reaction (PCR) was positive for all 18 strains of Kanagawa
phenomenon (KP)-Positive V. parahaemolyticus but negative for 6 strains of KP-negative
V.para-haemolyticus and the other 9 species (34 strains)of Genus Vibrio,and negative for
8strains of the other gram-negative bacteria. This PCR method could detected as few as 2CFU
of V.parahaemolyticus.
出处
《中国兽医学报》
CAS
CSCD
1996年第3期252-255,共4页
Chinese Journal of Veterinary Science
基金
军队青年科学基金
关键词
PCR
副溶血性弧菌
耐热溶血素基因
PCR
Vibrio parahaemolyticus
tdh gene Department of
Microbiology, The 302th Haspital of PLA
