摘要
目的:观察银杏叶提取物对蛛网膜下腔出血后氧自由基代谢的影响及该影响是否存在剂量依赖性。方法:①实验于2003-05/12在泰山医学院脑微循环实验室和附属医院中心实验室完成。选用Wistar大鼠54只。将动物随机分为5组:对照组(6只)、模型组(12只)、溶媒组(12只)、银杏叶提取物100mg/kg组(12只)和银杏叶提取物200mg/kg组(12只)。②股动脉采血后经冻-溶制备自体动脉血溶血物并注入大鼠枕大池制作蛛网膜下腔出血模型(除对照组外其余4组均造模)。③对照组:用0.3mL生理盐水代替动脉血溶血物行脑池注射;模型组:蛛网膜下腔出血造模后不给予干预;溶媒组:腹腔注射等体积含tween80的生理盐水;银杏叶提取物100mg/kg和银杏叶提取物200mg/kg组:在脑池注入自体动脉血溶血物开始前30min分别按100mg/kg和200mg/kg(首次剂量)腹腔注射银杏叶提取物溶液(为棕黄色粉末,由上海绿源制药有限公司提供,含银杏苦内酯≥6%,银杏黄酮≥24%。银杏叶提取物溶液在临用前新鲜配制,在银杏叶提取物粉剂中加入适量tween80溶液,研磨后,加入生理盐水使之成3%和6%的溶液),以后每天以上述半量重复2次;以上干预至各标本获取点前12h为止。④于造模后24h和72h按照购于南京建成生物工程研究所试剂盒说明书操作测定各组大鼠脑组织匀浆中超氧化物歧化酶活力和丙二醛含量。⑤计量资料差异比较采用方差分析。结果:大鼠54只均进入结果分析。①脑组织超氧化物歧化酶活性:模型组和溶媒组于造模后24和72h明显低于对照组(P<0.01);银杏叶提取物100和200mg/kg组于造模后24和72h明显高于模型组(P<0.01);银杏叶提取物100与200mg/kg组间差异不明显。②脑组织丙二醛含量:模型组和溶媒组于造模后24和72h明显高于对照组(P<0.01);银杏叶提取物100和200mg/kg组于造模后24和72h明显低于模型组(P<0.05～0.01);银杏叶提取物100与200mg/kg组间差异不明显。结论:银杏叶提取物能够有效减轻蛛网膜下腔出血后氧自由基连锁反应所致脑损伤,且不存在明显剂量依赖性。
AIM： To investigate the effect of ginkgo biloba （EGb） extract on metabolism of oxygenic free radicals after subarachnoid hemorrhage （SAH） and to observe the influence whether or not shows a dose-depended manner METHODS： ① The experiment was completed in the Microcirculatory Laboratory of Taishan Medical College and the Central Laboratory of Affiliated Hospital Of Taishan Medical College from May to December 2003. Fitly-four Wistar rats were used in the experiment and were divided into control group （n=6）, model group （n=12）, vehicle group （n=12）, 100 mg/kg EGb extract group （n=12） and 200 mg/kg EGb extract group （n=12）. ② The femoral artery was cannulated for blood withdrawing. The autologus arterial hemolysate was obtained by a freeze-meh method and then it was injected into the rat＇s cisterna magna to produce SAH models （SAH models were established in all groups except control group）. ③ Control group： The hemolysate was replaced by 0.3 mL saline. Model group： No intervention was given after SAH, Vehicle group： The same volume of saline containing tween 80 was injected intraperitoneally. 100 mg/kg EGb extract group and 200 mg/kg EGb extract group： The rats received intraperitoneal injection of EGb （100 mg/kg and 200 mg/kg respectively） 30 minutes before cisternal injection, and the injection was repeated twice daily with the half dose. EGb was brown-yellow power and provided by Lvyuan Pharmaceutical Co. Ltd, standardized on the amount of ginkgo bitter lactone ≥ 6%, flavone glycosides ≥ 24%. EGb was solubilized in a drop of tween 80 and physiological saline （3% and 6% respectively）. The stock solution of EGb was prepared just before use. Above interventions were not ceased until 12 hours after sampling. ④ 24 hours and 72 hours after cisternal injection, rats were sacrificed for determination of superoxide dismutase （SOD） activity and content of malondialdehyde （MDA） in the brain tissue homogenate. The manipulation followed by the instruction books of SOD ⑤ Measurement data were compared with analysis of variance. RESULTS： Totally 54 rats entered the statistical analysis. ⑥ SOD activities in brain tissue： Activities in model group and vehicle group were lower than those in control group 24 hours and 72 hours after modeling （P 〈 0.01）, but those in 100 mg/kg and 200 mg/kg EGb extract groups were higher than those in model group （P 〈 0.01）. The difference was not significant between 100 mg/kg EGb extract group and 200 mg/kg EGb extract group. ②MDA content： Contents in model group and vehicle group were higher than those in control group 24 hours and 72 hours after modeling （P 〈 0.01）, but those in 100 mg/kg and 200 mg/kg EGb extract groups were lower than those in model group （P 〈 0.05-0.01）. The difference was not significant between 100 mg/kg EGb extract group and 200 mg/kg EGb extract group. CONCLUSION： EGb may alleviate cerebral injury caused by oxygenic free radicals without obvious dose-depended effect after SAH.
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第7期28-30,共3页
Chinese Journal of Clinical Rehabilitation
基金
山东省中医药科研基金资助(2001-81)
山东省卫生系统高层次人才1020工程基金资助
国家自然科学基金资助(30570651)~~
