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MTT法探讨三氧化二砷及莪术对晶状体上皮细胞增殖的抑制作用 被引量:5

Study on the mechanism of proliferative inhibition of arsenic trioxide and rhizoma curcumae on lens epithelial cell by MTT assay
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摘要 目的探讨两种天然药物三氧化二砷(ATO)和莪术(RC)对晶状体上皮细胞(LEC)增殖的抑制作用,为寻求防治后发性白内障的确切有效药物提供实验依据。设计实验性研究。研究对象体外培养的牛LEC。方法倒置显微镜下观察细胞形态学变化。MTT比色法检测不同终浓度(2.5、5、10μmol/L)ATO和(5、10、20mg/ml)RC作用于增殖状态下LEC 72小时后对细胞增殖的抑制程度。主要指标细胞形态、吸光度(A)值。结果倒置显微镜下可见增殖对照组细胞大小均匀,生长密集,ATO和RC组细胞生长及形态有明显改变,增殖缓慢,生长差,贴壁能力减弱。MTT比色法结果显示:不同浓度(2.5、5、 10μmol/L)的ATO和(5、10、20mg/ml)RC可明显抑制LEC增殖,并具有剂量依赖关系(P均<0.01)。结论两种天然药物 ATO、RC能够明显抑制LEC增殖,有望成为防治后发性白内障的有效药物。 Objective To investigate the proliferative inhibition of lens epithelial cell(LEC) by arsenic trioxide (ATO) and rhizoma curcumae(RC), in order to provide scientific basis for pursuing safe and effective natural drugs to prevent and cure after cataract. Design Experimental study. Participants Cultured Bovine LEC in vitro. Methods Changes of cellular form were observed with microscope. Different concentration of ATO (2.5, 5, 10μmol/L) and RC(5, 10, 20mg/ml) were added to the proliferative LEC separately. The effects of proliferative inhibition by ATO and RC on the LEC were measured with methyl thiazolyl tetrazolium (MTr) assay method after 72 hours incubation. Main Outcome Measures Cellular form, Absorbance. Results Under the microscope, good growth and quantity increase were found in proliferative control group. Slowing proliferation,peor growth, and few disintegration were observed in ATO group and RC group. MTT assay: Different concentration of ATO (2.5, 5, 10μanol/L)and RC(5, 10, 20mg/ml) can significantly inhibit the proliferation of LEC and these effects were dose dependent(P〈 0.01 ). Condusion The inhibitive effects of ATO and RC on the LEC proliferation make them probably become reliable and effective drugs to prevent and cure after cataract. (Ophthalmol CHN, 2006,15: 46-49)
出处 《眼科》 CAS 2006年第1期46-49,共4页 Ophthalmology in China
基金 福建省卫生厅重点项目(2002503)
关键词 三氧化二砷 莪术 晶状体上皮细胞 碱性成纤维细胞生长因子 MTT法 arsenic trioxide rhizoma curcumae lens epithelial cell basic fibroblast growth factor , MTI
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