摘要
目的观察重组人生长激素(rhGH)在兔实验性角膜损伤修复过程中的作用。设计随机对照实验性研究。研究对象 40只实验用新西兰大白兔。方法将40只兔随机分成4组(每组10只),分别为4 IU／ml rhGH组,2 IU／ml rhGH组,贝复舒组(阳性对照组)及生理盐水组(阴性对照组)。将4组兔的右眼角膜中央用直径5．0 mm环钻制作成直径5．0mm、深度 0．1mm的无菌性角膜损伤。从术后第1天起各组分别点用4 IU／ml rhGH、2 IU／ml rhGH以及贝复舒、生理盐水。采用角膜荧光素染色、照外眼像。Matlab 6．5软件编程计算角膜损伤的面积。主要指标术后1-28天兔角膜损伤面积变化。结果术后3天生理盐水组(23307．9±22．1dpi)、2IU／ml rhGH组(10433．8±519．6dpi)角膜损伤面积与4IU／ml rhGH组(4838．7±192．3dpi)和贝复舒阳性对照组(7422．4±268．3dpi)有显著差异。4IU／ml rhGH组和阳性对照组促进角膜损伤面积减小效果相似,且优于2IU／ml rhGH组和阴性对照组。术后5天2IU／ml rhGH组角膜损伤面积减小到与4IU/ml rhGH组和阳性对照组无统计学差异,仅生理盐水组(8589．9±771.0dpi)与其它组在角膜损伤面积上有统计学意义的差异。结论重组人生长激素(rhGH)在兔角膜损伤修复的早期有明显促进作用。
Purpose To observe the effect of rhGH in the rehabilitation of rabbit corneal ulcer. Design Radomized, controlled experimental study. Participants 40 experimental melanin rabbits. Methods Forty rabbits were radomly divided into four groups （10 rabbits for each group）, that was high concentration 4 IU/ml rhGH group, low concontration 2 IU/ml rhGH group, Recombinant human （bovine） basic fibroblast group（positive control group）, and NS group （negative control group）. 0.1 mm depth sterile corneal ulcer was made in the center of right eye of all rabbit with 5 mm diameter trephine, Tobrex was usroup）, ed three times per day postoperatively. 4 IU/ml rhGH was used in the high concentration rhGH group, and 2 IU/ml rhGH was used in the low concentration rhGH group. Recombinant human （bovine） basic fibroblast was used in the positive control group, and 0.9%NS was used in the negative control group. All medication was used three times per day and continued for 28 days. Main Outcome Measures The rehabilitation of corneal ulcer of four group rabbits was observed and documented. Results rhGH had prominent facilitation effect of rehabilitation in corneal epithelium and superficial stroma, not only the heal up time of corneal ulcer were marked shortened in the high concentration rhGH group and the low concentration rhGH group compared with the negative control group, but also the high concentration rhGH group had better results in facilitation of ulcer healing and prohibition of ulcer recurrence compared with recombinant human （bovine） basic fibroblast which had affirmattery curative effect. Conclusion rhGH has marked facilitation effect in the healing course of rabbit＇s corneal ulcer. （Ophthalmol CHN, 2006, 15： 59-62）
出处
《眼科》
CAS
2006年第1期59-62,共4页
Ophthalmology in China
关键词
重组人生长激素
模型
动物
角膜损伤
recombinant human growth hormone
models, animal
corneal ulcer
