摘要
目的:应用cDNA芯片技术研究三氧化二砷对多发性骨髓瘤细胞株RPMI8226的作用。方法:采用包含4096个人类基因的cDNA表达谱芯片,检测三氧化二砷作用于RPMI8226细胞24h前后其基因表达的变化。结果:在mRNA水平上,273个基因的表达发生了明显改变,其中121个基因表达上调,152个基因表达下调。结论:三氧化二砷可引起RPMI8226细胞株一系列基因表达的改变。ZFYVE16、TXNIP及ALK1基因可能与RPMI8226细胞的分化与凋亡密切相关。
Objective： To compare the changes of gene expression profiles of multiple myeloma cell line RPMI 8226 before and after 24-hour intervention of arsenic trioxide. Methods： The responses of the RPMI 8226 cells to arsenic trioxide were determined with cDNA microarray which included 4 096 different human genes. Results： Of these 4 096 genes, the expressions of 273 genes were altered significantly at mRNA level, The expressions of 121 genes were up-regulated while the expressions of 152 genes were down-regulated, Conclusion： The effect of arsenic trioxide on RPMI 8226 cells is related to changing the expression levels of a number of genes. ZFYVE16, ALK1 and TXNIP genes may play important roles in apoptosis and differentiation of RPMI 8226 cells.
出处
《中西医结合学报》
CAS
2006年第2期160-165,共6页
Journal of Chinese Integrative Medicine
基金
陕西省科技攻关项目(No.2001KG9)
